GlycoSens™ Total Thiols Assay Kit, Spectrophotometry

The GlycoSens™ Total Thiols Assay Kit utilizes a highly stable detection system tailored for accurate profiling of total thiols. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Oxidation and Antioxidant Series

Colorimetric Reagent; Assay Buffer; Thiol Standard; Sample Diluent

Serum, plasma, tissue homogenates

10 μL - 50 μL

Target total thiols in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target total thiols.

Within 1 hour

412 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Functioning at the core of various biological processes, total thiols is a key subject of biochemical analysis. Its quantification is fundamental to establishing baseline profiles in biochemical and clinical assessments. Notably, monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.

Widely applicable for tracking total thiols fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.