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The GlycoSens™ Ferric Reducing Antioxidant Power (FRAP) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target ferric reducing antioxidant power in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Other Series
Kit Components
FRAP Buffer; TPTZ Chromogenic Solution; FeCl3 Solution; Standard Solution
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of ferric reducing antioxidant power. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect FRAP content in the sample.
Detection Time
Within 1 hour
Detection Wavelength
593 nm
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Application Notes
Background
Ferric reducing antioxidant power (FRAP) is recognized as a fundamental component in biological systems. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Monitoring these changes is vital for evaluating bioenergetic efficiency and systemic resilience.
Application
Designed for comprehensive functional analysis of ferric reducing antioxidant power (FRAP) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.