ATP-responsive Liposome Development Service

Creative Biolabs is at the forefront of ATP-responsive liposome technology, we can unlock the full potential of ATP-responsive liposome in the treatment of various diseases.

Background

Adenosine-5'-triphosphate (ATP) is a crucial biomolecule in cellular energy metabolism and signal transduction. A significant ATP concentration gradient exists between intracellular (1-10 mM) and extracellular (<0.4 mM) environments, which is considerably steeper than the pH gradient. This gradient is found in almost all cell types, yet tumor microenvironment may exhibit even higher ATP levels. Thus, a stimuli-responsive drug delivery system that capitalizes on the ATP concentration gradient can facilitate targeted cancer therapy.

Utilizing ATP as a stimulus offers several advantages:

• The steep intracellular/extracellular ATP gradient serves as an effective trigger, minimizing premature drug release prior to cellular uptake and enhancing intracellular drug accumulation.
• ATP's universal role as an energy transfer "currency", participating in various biological activities, making ATP-based responses a versatile stimulus-response strategy. Unlike ultrasound or light, it does not require additional equipment to trigger drug release.
• ATP levels can be modulated by other metabolic factors, such as glucose, expanding the regulatory mechanism's scope.
• The diverse interactions between drugs and ATP-responsive nanocarriers can be optimized for improved drug loading efficiency.

What is ATP-responsive Liposome?

ATP-responsive liposomes represent a novel class of stimuli-responsive liposomes that undergo structural changes upon binding to ATP, leading to the rupture of the lipid membrane and the release of encapsulated drugs. To confer ATP responsiveness to liposomes, functional modules that can distinguish ATP from other cytoplasmic components are required, which include the following two categories:

• Single-stranded DNA (ssDNA) aptamers with ATP specificity

These are chosen from a vast array of random ssDNA sequences for their high binding affinity to adenosine/ATP in vitro. With their relatively short sequences (around 30 bases), these aptamers are easy to functionalize and exhibit specific reactions, making them a popular choice for ATP-responsive liposome.

ATP-responsive liposomes are a novel class of liposomal delivery vehicles that are designed to undergo conformational changes upon binding to ATP, leading to the disruption of membrane packing and the subsequent release of encapsulated drugs.

Fig.1 Aptamer-based ATP-responsive liposome.

• Lipid switches designed to respond to ATP

Lipid switches are incorporated into the liposome formulation, designed to undergo significant conformational changes upon ATP binding, leading to structural degradation and the selective release of encapsulated drugs.

Fig.2 Lipid switch-based ATP-responsive liposome.

Advantages of ATP-responsive Liposome by Choose Creative Biolabs

• Targeted Drug Delivery: Our liposomes ensure the targeted release of drugs specifically in regions with elevated ATP levels, such as within tumors. This precision strategy significantly reduces the drug's distribution in healthy tissues, thereby diminishing systemic adverse effects.
• Diversity: These liposomes are designed to accommodate a broad range of cargo, encompassing small molecules, proteins, and nucleic acids.
• Robust Technical Platform: We leverage a state-of-the-art technical platform, equipped with advanced methodologies and expertise to optimize liposome formulation and production.
• Customizable Solutions: We pride ourselves on offering liposome formulations tailored to meet the unique requirements of each research project.
• One-on-One Technical Support: Our clients have direct access to our team of experts for personalized technical support.
• Comprehensive Quality Control (QC) System: We adhere to the highest standards of QC, ensuring that our liposome products meet the strictest requirements for safety, efficacy, and consistency.
• Data Security Assurance: We prioritize the protection of your research data, ensuring that all information is handled with the utmost confidentiality.

Creative Biolabs' expertise in ATP-responsive liposomes offers a cutting-edge solution for targeted drug delivery. Contact us to discuss how our ATP-responsive liposomes can be integrated into your research or treatment protocols.

For Research Use Only. Not For Clinical Use