Technologies for Glycan Profiling
In recent years, the research of glycoproteins has been receiving extensive attention. As a leader in this field, Creative Biolabs has been committed to the research of glycoproteins. Based on our precise knowledge and skilled technology, we are confident to help every customer around the world solve problems related to glycoprotein projects.
Technologies for Glycan Profiling
There are numerous glycan profiling methods, such as mass spectrometry (MS), lectin-based histochemistry, high-performance liquid chromatography (HPLC), multiplex quantitative PCR (qPCR), multiple reaction monitoring mass spectrometry (MRM-MS), and tandem mass spectrometry. Recently, a novel biological method-lectin microarray has been gradually developed. Although there are many techniques for the analysis of protein glycosylation, there is no universal method for quickly and reliably identifying glycan structures. Each type of technology has its advantages, and the choice of technology depends on factors such as the type of glycosylation present and the nature and quantity of the sample. Therefore, scientists might choose the best method or combination of methods for glycan analysis based on the information they wish to know.
Among the various methods used for determination, each profiling methodology has certain advantages in detecting glycan. Several typical methods and their advantages are shown in the table below:
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Techniques
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Advantages in Glycan Analysis
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MS
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A powerful tool for glycan structural analysis
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Widely used to detect unknown compounds of samples
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Can be used in combination with other technologies
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HPLC
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Separate and identify compounds in the sample
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Excellent resolution and ease of use
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Excellent reproducibility of repetitive separations
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qPCR
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Profiling the expression pattern of glycogenes
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Tandem mass spectrometry
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Identifying the glycan structure connected to the target glycoprotein
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lectin microarray
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Directly works on intact glycoproteins
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Sensitive and high-throughput
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Laser micro-dissection
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Target tissue sections of tumor specimens in a Retrospective manner
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MRM-MS
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Allow the site-specific glycosylation profile to be normalized to the absolute protein concentration
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We are proficient in every method, including but not limited to those listed above. Although each analytical method has certain advantages in detecting changes in proteoglycans, it is still difficult to clarify the detailed glycan structure only by relying on a single technique. For this reason, we have explored a variety of different combinations to meet different analysis needs. We can use a combination of different analytical methods to determine the glycan structures and better understand glyco-alterations on cell surfaces. In addition, we have also developed a variety of lectin-based microarray technologies, and the developed technologies have proven to be effective for expanding the application of lectin microarrays.
Based on a variety of superb technologies and an experienced team of experts, we have developed a series of glycan analysis technologies. We will choose the most suitable method according to your project. If you are interested in our services, please contact us for more information.
For Research Use Only.
Related Services:
- Glycan Profiling of Various Glycoprotein
- Glycan Component Classification
- Glycan Labeling
- Use Glycoenzymes to Understand Glycan Structure and Function
- Visualize Terminal Glycans Using Fluorescence or SDS-PAGE
- Glycans in Immunity
