What Are Glycosylated Oligonucleotides?
Glycosylation is one of the most promising strategies to enhance the performance of oligonucleotide-based drugs. Glycosylated oligonucleotides, including glycosylated DNA/RNA and antisense oligonucleotides (ASOs), offer improved biological stability, better cellular uptake, and modulated immunogenicity. Glycosylated oligonucleotides are synthetic nucleic acid molecules covalently linked to one or more carbohydrate moieties. Glycoengineered oligonucleotides exhibit altered physicochemical and biological properties, with increasing attention in gene regulation, antisense therapy, mRNA targeting, and vaccine development. Equipped with GlycoOptimize™ platform, Creative Biolabs provides comprehensive glycosylated oligonucleotide engineering services, designed to meet the growing demands of research.
Our Glycosylated Oligonucleotide Services
Creative Biolabs provides an integrated suite of glycosylated oligonucleotide services, customized to support your entire development pipeline:
Custom Glycosylation of Oligonucleotides
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Antisense oligonucleotide design using AI/ML tools
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Synthesis of mono- and poly-glycosylated oligonucleotides
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Site-specific glycosylation
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HPLC, MALDI-TOF MS, and LC-MS/MS characterization
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Glycan profiling and quantification
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Structural confirmation of glycosylation sites
Bioassay Development and Validation
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Cell-based uptake and functional assays
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Cytokine response and oligonucleotide immunogenicity assessment
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Stability and serum half-life testing
Workflow: From Design to Delivery
Our modular platform allows customizable service packages, from single-step glycosylation to full-cycle design and screening.
Fig.1 Oligonucleotide glycosylation workflow.
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Consultation & Target Analysis: Define goals & assess target biology
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Oligonucleotide Design: Custom design of oligonucleotide sequences
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Site-Specific Glycosylation: Precise glycan attachment at defined residues
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Analytical Validation: Verify modification integrity & profile
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Functional Assays: Test biological activity in vitro/in vivo
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Data Delivery & Support: Comprehensive results with expert follow-up
Applications of Glycoengineered Oligonucleotides
Antisense Therapeutics
Antisense oligonucleotide-based therapeutics and their applications against bacterial infections are being revolutionized by glycosylation. Glycosylation enhances uptake into infected tissues and modulates immune recognition, allowing for selective bacterial gene silencing, improved delivery in mucosal tissues and lower systemic immune activation.
siRNA and miRNA Therapeutics
Glycosylation enables selective targeting to hepatocytes, macrophages, and tumor cells through glycan-receptor recognition.
mRNA Stabilization and Delivery
Glyco-conjugation improves packaging into delivery vehicles like lipid nanoparticles and extracellular vesicles, while reducing degradation.
Vaccines and Immunotherapies
Glycosylated oligonucleotides can serve as immune adjuvants or carriers in vaccine formulations, promoting stronger and more targeted immune responses.
Why Choose Creative Biolabs?
With several years of experience in oligonucleotide glycoengineering, Creative Biolabs stands at the forefront of innovation in glycosylated nucleic acid design and development. Our service advantages include:
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End-to-end custom synthesis and conjugation platforms
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Rigorous QC and analytical validation
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Expertise in glycosylation for multiple biomolecules
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Dedicated support for every research project
Related Services at Creative Biolabs
Creative Biolabs delivers a comprehensive suite of glycoengineering solutions beyond oligonucleotides, enabling precision modification across diverse molecular platforms to meet specialized research needs:
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Custom Glycosylation of Expressed Proteins/Peptides
Site-specific glycosylation of recombinant proteins and peptides to improve solubility, bioactivity, and in vivo half-life.
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Glycosylated Antibody Engineering Service
Fc and Fab-region glycoengineering for modulating ADCC, CDC, and pharmacokinetics of therapeutic antibodies.
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Glycolipid Engineering Service
Design and synthesis of functional glycolipids for vaccine delivery, immune modulation, and membrane studies.
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Custom Glycosylation of Small Molecule Drugs
Sugar-conjugation of small molecules to improve pharmacokinetics, targeting, and metabolic profiles.
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Glycosylated Polymer Engineering Service
Glycan-functionalized polymers for drug delivery systems, biointerfaces, and biomaterials research.
Are you looking for time-saving and reliable methods that facilitate your research or product development? Our glycosylated oligonucleotide engineering services offer a cutting-edge solution to enhance the stability, targeting, and efficacy of your oligonucleotides. Don't let your projects be limited by conventional approaches. Contact us today for a consultation and discover how our expertise in glycosylated oligonucleotide engineering can help you achieve your goals. Let's discuss your specific needs and provide a tailored quote that brings your vision to life!
FAQs
Q: Is glycosylation applicable to RNA therapeutics?
A: Absolutely. Glycosylated siRNA and mRNA show enhanced pharmacokinetic profiles and cellular uptake.
Q: What types of glycans can be used in oligonucleotide modification?
A: Both N- and O-linked glycans, including mannose, galactose, sialic acid, and custom glycan structures.
Q: Is glycosylation compatible with other oligonucleotide modifications?
A: Yes, it can be combined with PEGylation, lipidation, or fluorescent tagging.
Reference
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Khuu, Alexandre, et al. "Clinical Applications of Antisense Oligonucleotides in Cancer: A Focus on Glioblastoma." Cells 13.22 (2024): 1869. https://doi.org/10.3390/cells13221869
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