Custom Anti-GM2 Antibody Service for Gangliosidosis Research

Ganglioside GM2 Overview

Ganglioside GM2 is a sialylated glycosphingolipid enriched on neuronal membranes, especially within lipid rafts—microdomains that choreograph receptor signaling and axonal stability. In GM2 gangliosidosis (Tay–Sachs and Sandhoff models), lysosomal catabolism stalls and GM2 accumulates across compartments. For researchers, a rigorously profiled anti-GM2 antibody becomes a practical lens to quantify GM2 pools, visualize subcellular trafficking, and evaluate whether gene/enzyme interventions truly move the biochemical needle.

Fig.1 Pathophysiology of GM2 gangliosidoses.¹

The hard part isn't raising an antibody—it's proving what it really binds in membranes. Carbohydrate headgroups of GM1, GD1a, and GM2 are closely related, and when gangliosides cluster in rafts they can create ganglioside-complex (GSC) epitopes that don't exist on single molecules. Without testing in lipid context, a clone that looks "GM2-specific" on plastic can light up unintended complexes in tissue, driving false conclusions. That's why membrane-aware validation isn't optional; it's the guardrail that keeps your data interpretable. At Creative Biolabs, we offer a suite of custom anti-ganglioside antibody solutions for our clients to meet different research needs, including custom anti-GM2 antibody service—end-to-end design, engineering, and lipid-context validation tailored to your assays. Or tap our off-the-shelf anti-GM2 antibodies for immediate RUO workflows.

Design Custom Anti-GM2 Antibody for You

Creative Biolabs designs, generates, and qualifies custom antibodies to GM2 for advanced discovery programs. You can select:

• Formats: mouse/rat/rabbit monoclonal antibody, recombinant IgG (IgG1–IgG4), scFv, Fab, human/rhesus-framework chimeras; labeled or unlabeled.
• Use cases: ELISA, lipid-ELISA, IF/IHC, flow, Western, and SPR/BLI.
• Specificity paneling: exhaustive ganglioside panels (GM2, GM1, GM3, GD1a, GD1b, GT1b, GQ1b) plus mixed ganglioside-complex formats to expose clustered conformational epitopes; cross-reactivity triage is mandatory for credible GM2 claims.
• Biophysical epitope mapping: STD-NMR ± SPR to confirm atomic-level contacts that differentiate GM2 from look-alike glycans.

Start Your Project

Concise Anti-GM2 Antibody Development Workflow

Our streamlined workflow for anti-GM2 antibody development ensures high specificity and performance. The process begins with antigen preparation, where high-purity GM2 is synthesized and conjugated to carrier proteins (e.g., KLH, BSA) or integrated into liposomes to enhance its immunogenicity. Next, in the immunization phase, host animals like mice or rabbits are immunized using an optimized strategy with adjuvants to elicit a potent, GM2-specific B-cell response. For antibody screening and production, we offer polyclonal antibody isolation from serum or generate superior monoclonal antibodies using advanced platforms such as hybridoma, phage display, or single B-cell cloning technologies. The critical antibody validation step confirms specificity and affinity via ELISA and rigorously assesses cross-reactivity using glycan arrays to eliminate off-target binding. Finally, successful clones undergo large-scale production and are purified through affinity chromatography to deliver high-purity, active antibodies for research needs.

Fig.2 The workflow for anti-GM2 antibody development.

Ready-to-use Anti-GM2 Antibody Products

If you need an off-the-shelf antibody to get a quick start, Creative Biolabs also provides you ready-to-use anti-GM2 antibody products that are suitable for diverse applications.

Why Creative Biolabs

• Reliable glycolipid screening. We combine glycan microarrays with lipid-embedded assays to confirm specificity under membrane-like presentation—critical for gangliosides.
• Complex-epitope risk control. We test ganglioside-complex pairs to surface conformational epitopes otherwise missed in single-antigen screens.
• End-to-end execution. From immunogen to report, including humanization/chimerization and recombinant supply.
• Application-anchored validation. IF/IHC on neural tissues, flow with GM2-liposomes, and orthogonal ELISA formats to reduce false positives.

Research Applications

• GM2 gangliosidosis models. Track GM2 loads, subcellular redistribution, and rescue kinetics in gene-editing or enzyme-augmentation studies.
• Neuropathy & cross-reactivity studies. Anti-ganglioside responses can target complexed epitopes; anti-GM2 antibody tools help probe mechanistic links and specificity versus related antigens.
• Lipid raft biology. Gangliosides cluster in rafts where carbohydrate headgroups form conformational epitopes—your antibodies must be proven selective in this environment.
• Assay development. Build diagnostic antibody-style assays (research-grade) with stringent cross-panel controls before clinical assay translation is considered externally.

Popular Services You May Be Interested in

• Custom Anti-GM1 Antibody Development (for research)
• Glycosphingolipid (GSL) Panel Screening—GM1/GM2/GD1a/GD1b/GT1b/GQ1b arrays and complexes.
• Glycosphingolipids Analysis Service
• Glycan Microarray Profiling

Are you ready to design your anti-GM2 program? Tell us your target format and assays. We'll respond with a specificity-first plan to deliver a high-specificity anti-GM2 antibody suitable for demanding ganglioside GM2 research—whether you need a diagnostic antibody prototype for RUO assays or an engineered backbone fit to explore therapeutic antibody concepts (preclinical only). Contact our scientists now to start a quick consultation.

FAQs

Reference:

1. Leal, Andrés Felipe, et al. "GM2 gangliosidoses: clinical features, pathophysiological aspects, and current therapies." International journal of molecular sciences 21.17 (2020): 6213. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/ijms21176213

Supports

• Glycolipid