Microbial Glycan Antigen Microarray
Background: The Diversity of Bacterial Surface Glycans
The surface of bacteria contains numerous complex and varied carbohydrate structures. These surface glycans, including lipopolysaccharides (LPS), capsular polysaccharides, and glycoproteins, play essential roles in bacterial survival, colonization, and interaction with the host. Among the most well-known bacterial surface glycans are the lipopolysaccharides of Gram-negative bacteria. LPS is composed of lipid A, a core oligosaccharide region (commonly divided into inner and outer core), and the O-antigen, which is highly diverse between species and even strains of the same species. Gram-positive bacteria, on the other hand, are characterized by a thick peptidoglycan layer, which is often accompanied by teichoic acids or lipoteichoic acids with varying structures. Mycobacteria, the causative agents of tuberculosis and leprosy, have a distinct cell wall composition that includes peptidoglycan, arabinogalactan, and mycolic acids. This group also produces additional unique glycans such as lipomannan and trehalose-containing lipids. The incredible diversity of bacterial surface glycans contributes to their pathogenicity and ability to evade host immune responses. Understanding and characterizing these glycans are crucial for developing effective diagnostic tools, therapeutics, and vaccines against bacterial infections.
What Is a Microbial Glycan Antigen Microarray?
A microbial glycan antigen microarray (MGM) is an array of bacterial glycan antigens (often O-antigens) covalently bound to a solid support slide, against which one or many biological samples are interrogated in parallel to profile sample binding preferences and specificities. Microbial glycan antigen microarrays can contain either synthetic or isolated microbial glycans that span a wide diversity of glycan structures from known pathogens. Microbial glycans are synthesized or isolated from bacteria and derivatized and printed on solid support slides in microarray format for interrogation of a wide variety of biological samples including but not limited to antibodies, serum, whole cells, viral particles, and other glycan-binding molecules or cells.
Fig.1 The role of innate immune lectins through bacterial glycan microarrays.1
Design Your Microbial Glycan Antigen Microarray at Creative Biolabs
At Creative Biolabs, we understand that every research project is different. That's why we offer customized glycoarray solutions tailored to your unique needs. Our customizable microarray services are a great choice if you want to focus on particular bacterial strains, O-antigens or glycan epitopes of interest to your project.
Customize with us
Creative Biolabs provides customizable glycoarray services that can be adapted to suit your research project's unique needs. With us, you have the flexibility to select specific microbial glycans, including microbial glycans synthesized by us or isolated from bacterial cultures, and incorporate them into our microbial glycan antigen microarray format. Our state-of-the-art printing technology and NHS-activated glass slides enable high-density immobilization of glycans with precise spot size and placement control, ensuring reliable and reproducible results. Customization at Creative Biolabs doesn't stop there. We also offer flexibility in the assay detection method. We are pleased to offer a variety of customizable microarrays for your research, starting with the selection of glycan antigens or glycoproteins, bacterial strains, sample choice, and the number of subarrays you need, from single and multiple to high-density subarrays for the most optimal testing of your samples. With minimal sample volume, you can gain actionable insights using our customized microarray platform.
Customization Features
Creative Biolabs offers you the choice of selecting your own O-antigens for your research projects, providing an option for a customized MGM platform as needed. You are free to choose from a range of O-antigens, offering flexibility for various experimental needs. Customization extends to the ability to focus on specific bacterial strains or glycan motifs of interest tailored to your research objectives.
- Select Your Glycans: Choose from a wide array of bacterial surface glycans, including O-antigens, lipopolysaccharides, and other polysaccharides.
- Choose Suitable Formats: Choose from single, multiple, or high-density subarrays for optimal sample testing.
- Get Fast Results: Get fast, actionable insights with our high-performance microarrays and with minimal sample volume.
Simple Guide
Creative Biolabs offers a variety of microarray solutions to meet your research needs, and we are always here to help you choose the best platform for your specific study, here is a simple guide for the best-fit microarray selection:
- Antibody microarrays are best suited if you're focused on detecting bacterial infections or identifying bacterial strains using antibodies.
- Lectin microarrays are ideal if you need to profile bacterial glycans or differentiate strains based on their surface sugars.
- Cell wall fragment and whole cell microarrays are perfect if you're interested in how immune cells or proteins interact with live bacteria or cell wall structures.
- Bacterial glycan microarrays are the go-to choice if you're exploring microbial glycan diversity, glycan-binding proteins, or need to develop glycan-based vaccines.
Are you want to know more details about different microarrays for bacterial surface glycan research? Or still unsure about which type of microarrays is the most suitable one to use for your project? Do not worry, our experts are here to help! Describe your project in details and just wait for our reply.
Discuss Your Project Now
Key Advantages of Our Microbial Glycan Microarray
- High sensitivity and specificity for microbial glycan interaction
- Customizable microarrays to suit specific research needs
- Fast and efficient with rapid results
- Minimal sample volume required for high-throughput analysis
Creative Biolabs provides state-of-the-art microbial glycan analysis services and customized microarray solutions for researchers worldwide. Whether you are interested in characterizing pathogen-specific antibodies, profiling glycan-binding proteins, or screening for potential vaccine candidates, our microbial glycan antigen microarray platform offers the reliability and versatility you need for your research. Contact us today to learn more about our microbial glycan arrays and the many ways they can support your projects.
Reference:
- Campanero-Rhodes, María Asunción, et al. "Microarray strategies for exploring bacterial surface glycans and their interactions with glycan-binding proteins." Frontiers in Microbiology 10 (2020): 2909. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fmicb.2019.02909