Custom Anti-GM3 Antibody Service for Cancer & Metastasis Studies

GM3 Background

Ganglioside GM3 has been termed as the "entry point" ganglioside. This is in no way an understatement. As the simplest of the ganglioside family with a single sialic acid linked to lactosylceramide, GM3 has several important functions, such as the organization of lipid rafts, modulation of receptor clustering, and tuning of signaling pathways that control cell proliferation, migration, and intercellular communication. In the context of cancer, one specific variant of GM3 is of particular interest to researchers: Neu5Gc-GM3 (NGcGM3). Humans cannot make Neu5Gc naturally, yet it can build up in tumors through dietary incorporation. In fact, it is nearly undetectable in healthy tissues, which makes NGcGM3 a particularly attractive cancer biomarker. Structural insights from the well-studied antibodies 14-F7 and chP3 reveal the depth of the pocket in which the Neu5Gc residue is "locked in", completely excluding Neu5Ac. This level of pocket-mediated recognition is part of what makes GM3 a strong target for cancer immunotherapy and metastasis studies, as well as biologically fascinating. At Creative Biolabs, we use these types of insights to design anti-ganglioside antibodies. With years of glycoimmunology and antibody engineering experience, our team is well-versed in custom antibody design against complex glycans such as GM3. We use antigen design techniques, multi-platform screening, and glycan array specificity mapping in the design process, in order to provide clients with the most specific antibodies possible. For biomarker validation studies, mechanistic analyses, preclinical cancer research, and more, our scientific team and technical support can help speed your research forward. We also provide ready-to-use anti-GM3 antibodies for your quick research.

Fig.1 GM3 ganglioside species in serum showing acyl-chain structures.¹

Custom Anti-GM3 Antibody Services at Creative Biolabs

We provide end-to-end custom antibody generation against GM3 and its clinically relevant variants (NGcGM3 and NA-GM3), across monoclonal, recombinant, and engineered formats. Programs are built for cancer biomarker discovery, mechanism interrogation, and translational assay development in cancer immunotherapy pipelines. Core modules include immunogen design (synthetic glycan–carrier and liposomal display), discovery by hybridoma/yeast or phage display, multi-tier specificity screening (glycan array, TLC-overlay, cell-based), affinity maturation and humanization, and RUO assay development (IHC/IF, ELISA, FC, SPR/BLI).

GM3 Services Highlights at a glance

• Epitope-focused antigen design delivers GM3 variants in optimized liposomal or carrier-conjugated formats, ensuring antibodies with maximum selectivity.
• Versatile antibody formats are available, including monoclonal, recombinant, and engineered types such as scFv, Fab, and full-length IgG1/IgG4, tailored for oncology-focused RUO studies.
• Integrated discovery platforms combine hybridoma and phage/yeast display technologies, capturing diverse clones with high affinity and specificity.
• Rigorous specificity profiling is performed using glycan arrays, TLC-overlay, and cell-based assays against structurally related gangliosides (GM1, GM2, GD2, GD3, asialo-GM1).
• Deep-pocket recognition strategies are applied, modeled after structural insights from 14-F7 and chP3 antibodies, to achieve precise Neu5Gc discrimination.
• Broad functional applications you can choose for cancer biomarker analysis and cancer immunotherapy research, such as ELISA, IHC/IF, flow cytometry, and SPR/BLI.
• Comprehensive deliverables include antibody sequences, expression vectors, stable cell pools, purified material, and detailed SOPs for seamless integration into client workflows.

What You Receive

• Antibody assets: hybridoma or recombinant clone; sequences; expression vectors; stable pools; small-scale purified RUO antibody.
• Validation package: antigen maps; glycan array graphs; cross-reactivity matrix; binding kinetics; cell-based validation; IHC/IF or FC protocols.
• Transfer docs: SOPs for assay setup; recommended controls; storage & handling.

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Ready-to-use anti-GM3 antibodies

Need immediate tools? Explore our ready-to-use anti-GM3 antibodies products and technical datasheets:

Why Choose Creative Biolabs

• Focused expertise. Several years dedicated to glycobiology and anti-ganglioside antibody programs.
• Precision at every step. From antigen design to glycan arrays, our workflow is built to guarantee specificity.
• Cancer-aligned. Services tailored for biomarker discovery and cancer immunotherapy research.
• Seamless support. End-to-end delivery—from concept to validated antibodies with full data packages.

Applications in Oncology

Popular Services You May Be Interested in

• Anti-GM1, Anti-GM2, Anti-GD2/GD3 Antibody Services
• Glycan Microarray Profiling
• Antibody Humanization & Engineering (maintain deep-pocket recognition)

Design a precision GM3 antibody with data that holds up in oncology labs. Share your target and model system—our team will return a tailored plan aligning immunogen chemistry, discovery route, and validation assays to your cancer immunotherapy and cancer biomarker goals. Contact us for further information.

FAQs

Reference:

1. Inokuchi, Jin-ichi, and Hirotaka Kanoh. "Pathophysiological significance of GM3 ganglioside molecular species with a particular attention to the metabolic syndrome focusing on toll-like receptor 4 binding." Frontiers in Molecular Biosciences 9 (2022): 918346. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fmolb.2022.918346

Supports

• Glycolipid
• GM3 Antibody in Cancer Immunotherapy
• GM3 Ganglioside in Disease & Anti-GM3 Antibody Tools