With integrated antibody development and engineering techniques, Creative Biolabs offers a comprehensive set of anti-glycan antibody services for our global clients. We provide either individual service modules or a full antibody development service. Now, we tailor services to develop high-quality anti-LacNAc antibodies for multiple applications.
Poly-LacNAc (Poly-N-acetyllactosamine) comprises repeated Galβ1-4GlcNAc disaccharides, called N-acetyllactosamine (LacNAc). The biosynthesis of poly-LacNAc is accomplished by the enzymatic action of β-1,3 N-acetylglucosaminyltransferase (β3gnt) which catalyzes the addition of GlcNAc to N-glycan Gal termini. Among the several homologs of β3gnt, β3gnt2 is crucial enzyme for synthesis of poly-LacNAc in CHO cells. Poly-LacNAc structures occur in mammalian glycoproteins in both N- and O-linked glycans. They represent a backbone for additional modifications by sialyltransferases, fucosyltransferases, and sulfotransferases. Poly-LacNAc could serve as a potential age-specific ligand for attachment and infection of Rotavirus P in neonates and infants.
Fig.1 Schematic structure of a typical poly-LacNAc type N-glycan. (Zhou, 2003)
Poly-LacNAc in tumor growth and metastasis is the most attractive aspect regarding its biological significance. One leading mechanism is that the N-linked poly-LacNAc glycans are supposed to provide a backbone for attachment to adhesion molecules, which trigger the signaling pathways for tumor cell growth and invasion.
TCR complex at the cell surface was associated with galectins, which are known to modulate T-cell proliferation and apoptosis. The association is related to β1, 6 branched poly-LacNAc on N-glycans.
It is known that oligosaccharides serve as specific receptors for bacteria, viruses, and parasites. Some of these glycoconjugates contain poly-LacNAc sequences.
The decoration of erythrocyte membrane anion transporter by poly-LacNAc is important for membrane stability. The lack of poly-LacNAc leads to aggregation of the membrane glycoproteins and abnormal membrane structures.
In the study on the protozoan parasite, Trypanosoma brucei, the N-linked glycans containing linear poly-LacNAc were recognized as sorting signals for membrane proteins in endocytosis.
The poly-LacNAc is one of the most challenging structures for both glycochemists and glycobiologists. Cell lines that have characteristic expression pattern of glycosyltransferases might be used by the combined transfection of key enzymes involved in the poly-LacNAc synthesis. Creative Biolabs is equipped with state-of-the-art research and manufacturing facilities and dedicated to help our clients design and prepare highly customized anti-LacNAc antibody using our featured services. With novel technology platform and professional experiment services, Creative Biolabs gets ready to provide you with the best antibody services. Please feel free to contact us for more details.
Reference:
