Genetically engineering models are animals that bear a genetic alteration in the DNA, which are important and powerful tools for research on human diseases as well as to develop therapeutic interventions. An ideal model on a defined genetic background can always be the cornerstone of your research. Here at Creative Biolabs, we offer custom tailored models to meet every specific need of our clients. Having a group of highly-experienced custom model generation specialists and equipped with conventional and the latest technologies, Creative Biolabs, is capable of providing you with the optimal genetically engineered model to your exact expectations.

Genetically Engineering Models (GEMs)

Generation of GEMs through Multiple Approaches

Model Creation Technology

Model generation technologies available at Creative Biolabs involve both traditional and newly developed methods. Further, genetic mutations can be achieved in zygotes and ES cells with various gene-editing techniques, including DNA microinjection and transposon-mediated genome manipulation, as well as designer nucleases such as Transcription Activator-Like Effector Nucleases (TALENs) and the RNA-guided nuclease system called the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR). These choices of technologies guarantee that models are fast and efficiently generated and delivered.

Knock-out Models

Gene knock-out is the manipulation of the DNA that results in the nonfunctionality or complete loss of the corresponding protein. Knock-out models refer to ‘loss of function' mutants. Typically, they can be divided into three types: conventional knock-out models, conditional knock-out model, and KO first (conditional ready) models. In conventional KO models, the targeted gene is inactivated in all tissues at all times, while it is inactivated in specific organs at specific time points in conditional KO models. In, KO first models, the target region is flanked by loxP sites ("floxed") to facilitate Cre recombinase-mediated excision.

Knock-in Models

The integration of DNA or gene of interest can be inserted randomly or at a specific site based on distinct techniques, resulting in the generation of ‘gain of function' mutants. Randomly transgenic models can be obtained via transposon-mediated insertion, DNA microinjection, and lentivirus-mediated transfection methods. Knock-in models have a genetic element inserted into a specific locus in the genome. The targeted introduction of a foreign gene into embryos or ES cells can be achieved in such forms as point mutation and cassette insertions (eg. Reporter gene).

Knock-down Models

Gene knock-down is the technique used to reduce the expression of one or more of an organism's genes, resulting in temporary function neutralization. This can be achieved by genetic modification or by the use of a short RNA oligonucleotide such as siRNA, which is complementary to the target mRNA to be silenced.

Humanized Mouse Models

Humanized mouse models refer to mouse models which are engrafted functional human genes, cells or tissues, thus are perfect model systems for in vivo studies of human diseases and for therapeutic improvement. Genetically humanized mice from Creative Biolabs cover a wide range of mice types, from immunodeficient humanized mice models to immune checkpoint knock-in models.

Our Advantages in GEMs Generation

  • Animals breeding under VAF Elite/SOPF conditions
  • Experiments performed in a GLP-compliant and IACUC-regulated facility
  • Expertise in not only model generation but also project assessment and data analysis
  • Personalized support from before the project initiates to after models are delivered
  • An accurate timeline of the entire development process

With the support of cutting-edge technologies and specialists in this field, Creative Biolabs is proud to offer the most reliable genome engineering model services at most favorable prices currently on the market. Please feel free to contact us to discuss the ideal custom mouse model to boost the impact of your next project and beyond.

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