Creative Biolabs is one of the most reliable and professional industry leaders in developing animal models of diseases. In particular, we provide air pouch models induced in rats and mice for preclinical research of anti-inflammatory drugs screening and in vivo efficacy tests.
Introduction of Air Pouch Model
The air pouch is developed simply by subcutaneous (s.c.) injection of sterile air into the intra-scapular area of the back of a rat or mouse. This procedure induces the proliferation of cells that stratify on the surface of the cavity to form a cellular lining consisting of macrophages and fibroblasts, which is morphologically similar to that found in synovial cavity. Later, local inflammation can be induced by injecting with carrageenan solution or other irritants (e.g. LPS, zymosan or bradykinin) into the air pouch. Injection of a carrageenan solution into the pouch produces an inflammatory response that is characterized by:
Here, the pouch serves as a reservoir of locally accumulated cells and mediators that can be easily measured in the fluid. These endpoints can be quantified and used to determine the degree of inflammation, resolution of inflammation, or anti-inflammatory activity of drugs.
Applications of Air Pouch Model
The subcutaneous air pouch is an in vivo model used to study acute/chronic inflammation, granulomatous inflammation, the resolution of the acute inflammatory response, and evaluation of oxidative stress. In preclinical studies, it is frequently used to screen potential anti-inflammatory compounds.
Advantages of Air Pouch Model
There are several advantages of the air pouch over the injection of irritants directly into the knee joint of animals: Firstly, the volume of exudate produced in the air pouch is much larger, enabling the measurement of multiple parameters from the same animal. Secondly, air pouch model is much easier to manipulate because it is technically challenging to perform procedures including direct injection of irritants to a knee joint and subsequent collection of inflammatory exudates without causing damage leading to inflammation.
Creative Biolabs provides different parameters that can be used to evaluate the extent of inflammation and the effect of a given test compound, including but not limited to:
Creative Biolabs also offers other rodent inflammatory & immunological disease models listed as follows that you may be interested in:
At Creative Biolabs, experienced project teams are assigned to each study focusing on progressing projects through to results in the minimum amount of time. To get more information, a quote or to schedule a teleconference please contact us. Besides, if you have a specific need then contact us to discuss your requirements.
For Research Use Only.
