Creative Biolabs offers a battery of rodent models including chemically- and surgically- induced OA models for studies of disease mechanisms as well as the testing of therapeutic candidates. We have talented experts and scientists who are willing to help to set up a detailed research plan based on your specific needs. Herein, you can find the most comprehensive services with the most reasonable prices.
Chemically-Induced OA Models
Osteoarthritis (OA) is a complex disease process involving the whole synovial joint. Animal models of osteoarthritis have greatly improved our understanding of its pathogenesis and treatment. Chemically-induced OA models mostly involve the injection of a toxic or inflammatory compound directly into the knee joint to stimulate intra-articular inflammation, direct matrix damage, or chondrocyte toxicity. Papain, monosodium iodoacetate, quinolone, and collagenase are some of the chemicals commonly employed to induce OA in animals. They eliminate the need for surgery and avoid possible infection issues in some animals. Their ease of induction and reproducibility are advantageous in designing short-term studies.
Monosodium Iodoacetate (MIA)-Induced OA Model
A well-recognized method to induce OA is the intra-articular injection of monosodium iodoacetate (MIA), which is an inhibitor of glyceraldehyde-3-phosphate dehydrogenase activity shown to induce chondrocyte death and hence reproduces cartilage lesions with loss of proteoglycan matrix and functional joint impairment that closely resemble conditions seen in human OA. Despite the fact that chemically-induced models may not replicate either spontaneous or posttraumatic OA progression, they are useful as translational the animal model of OA in both mechanism study of the pain and as a target for drug therapy due to the below inherent advantages:
OA Induction
OA is induced by a single intra-articular injection of MIA dissolved in saline solution into the right knee joint of anesthetized rats or mice. Normal control animals are injected with an equal volume of saline. Left knee remains un-injected. In testing the therapeutic efficacy of drug candidates, we are capable of conducting all kinds of assessments as well as providing assistance in data interpretation.
Fig.1 Effect of vitamin C on articular cartilage of rat with MIA-induced OA. (Chiu et al. 2016)
Our assessments including but not limited to:
Additionally, other examples of rodent musculoskeletal disease models that you may be interested include:
With years of experience in studies of disease animal models, scientists from Creative Biolabs are willing to work with you for a custom design to meet your specific requirements. Please feel free to contact us or send us an inquiry, if you are interested in any of these services.
Reference
For Research Use Only.
