“Humanized” mouse model of immunity refers to normal, immunocompetent mouse expressing human genes by transgenesis (such as HLA or human immunoglobulin transgenic mice) or to immunodeficient mouse engrafted with human hematopoietic and lymphoid cells or tissues. Immunodeficient mice engrafted with human cells/tissues have provided a potential approach for the study of human immunobiology. The humanized SRC-SCID model is established by injecting newborn or adult immunodeficient mice with HSCs from a variety of sources and has been regarded as a supreme in vivo model for long-term studies in the fields of immuno-oncology, infectious disease, and graft versus host disease. With years of experience in animal model, Creative Biolabs can provide the validated humanized SRC SCID mice for our worldwide clients to evaluate the safety and efficacy of potential novel agents to modulate the immune system.
Humanized SRC SCID Mouse
The discovery of the severe combined immunodeficiency (SCID) mouse mutation has provided a useful tool to establish small animal models as hosts for the in vivo analysis of normal and malignant human pluripotent hemopoietic stem cells. Intravenous injection of irradiated SCID mice with human bone marrow, cord blood, or G-CSF cytokine-mobilized peripheral blood mononuclear cells which are all rich in human hemopoietic stem cell activity, leads to the engraftment of a human hemopoietic system in the murine recipient. This kind of mouse model has been used to identify a pluripotent stem cell, termed “scid-repopulating cell” (SRC) that is more original than any of the hemopoietic stem cell populations identified using the currently available in vitro methodology.
The major advantage of the HSC engraftment model (humanized SRC-SCID) is that the human T and B cells develop from human stem cells engrafted in the mouse, undergo negative selection during differentiation into T and B cells, and are consequently tolerant of the mouse host. The humanized SRC SCID mouse model permits the investigation of hematopoietic lineage development and mechanisms of immune system development and the generation of primary immune responses by a naïve immune system.
Establishment of Humanized SRC SCID Mouse
Fig. 1. Approaches for the engraftment of human immune systems in immunodeficient mice. To establish Hu-SRC-SCID (human SCID-repopulating cell SCID) mice, haematopoietic stem cells (HSCs) are injected into adult recipients via an intravenous or intrafemoral route. Injection routes into newborn mice include the facial vein route, and intracardiac and intrahepatic injection. Intraperitoneal injection of HSCs into newborn or adult recipients results in very low engraftment. (Shultz et al., 2012)
Features
The humanized SRC-SCID system has been applied in investigating many aspects of the human hemopoietic system, to study the growth of human leukemias in vivo and to analyze heritable hemopoietic disorders. The humanized SRC-SCID system has also been extended to investigate human stem cells present in tissues other than bone marrow. Creative Biolabs, a world leader in the industry of animal model, has established an array of humanized SRC SCID mouse models that are verified by advanced technology and these models are available for delivery or being incorporated into our drug efficacy testing services.
As a world leader in the industry of animal model, Creative Biolabs also offers other Humanized Mouse Models you may be interested in:
If you are interested in learning more about Creative Biolabs' animal models or drug discovery service, please contact us for more details.
Reference
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