Kidney Transplantation Modeling & Pharmacodynamics Services
Creative Biolabs offers a variety of well-established rodent kidney transplantation models for preclinical research. These models are designed to closely mimic the human transplant process, including kidney graft function, immune responses, and rejection processes. Our models enable the comprehensive evaluation of new immunosuppressive drugs, organ preservation techniques, and strategies to prevent transplant rejection.
Introduction
Kidney transplantation is a critical treatment for patients suffering from end-stage renal disease. Preclinical research on kidney transplantation models is essential for assessing transplant rejection, immune response, graft survival, and therapeutic efficacy of immunosuppressive agents. Kidney transplantation models can be divided into allogenic and autologous models, as well as syngeneic models, each simulating different aspects of human transplantation. Allogeneic models involve transplanting a kidney from a genetically different donor to the recipient, while autologous models use the recipient's own kidney. Syngeneic models involve transplanting a kidney between genetically identical animals.
Kidney Transplantation Models
Creative Biolabs offers a wide range of well-established rodent models for kidney transplantation research, including models for allogeneic, autologous, and syngeneic kidney transplants. These models are meticulously designed to replicate human kidney transplantation and allow for the evaluation of various post-transplantation challenges, such as graft rejection, immune response, and drug efficacy in preventing organ rejection. Our kidney transplantation models are carefully optimized to simulate human clinical conditions and come with detailed assessments of key parameters, including graft function, histological analysis, immune infiltration, and cytokine profiles. Our experienced scientists will work closely with you throughout the process, from experimental design to data interpretation, ensuring reliable and high-quality results. Explore the links below to learn more about our rodent kidney transplantation models available for preclinical research.
Fig. 1 The protocol of a rat kidney transplantation model.1
| Kidney Transplantation Model | Simulates | Evaluates Drugs | Animal species |
| Rat Warm Kidney Graft Transplant Model | Acute kidney transplant rejection and ischemia-reperfusion injury | Immunosuppressants, anti-rejection drugs, ischemia-reperfusion injury protectants | Rat |
| Rat Delayed Kidney Graft Transplant Model | Delayed graft function, kidney transplant rejection, and acute kidney injury | Immunosuppressants, anti-inflammatory agents, and drugs improving delayed graft function and kidney recovery | Rat |
Evaluation Platform
- Animals: Rat.
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Measurements
We offer a variety of measurements for evaluating drug efficacy in rodent kidney transplantation models, utilizing advanced technologies, including but not limited to:- General Observations: Body weight, mortality rate, kidney function, and graft survival.
- Histopathology: Kidney graft histological evaluation, including inflammation, fibrosis, and immune cell infiltration.
- Immunohistochemistry: Detection of immune cell infiltration (e.g., T-cells, macrophages) in transplanted kidney tissues.
- Cytokine Profiling (e.g., ELISA): Expression levels of inflammatory mediators such as TNF-α, IL-6, and IL-1β in serum or tissue samples.
- Hematology Analysis and Serum Biomarkers: Kidney function markers, including creatinine, BUN, and uric acid levels.
- Gene/Protein Expression Profiling: Quantitative PCR and Western blot techniques to assess immune-related gene expression and protein markers of graft rejection.
Additionally, our team can develop novel models tailored to your specific research needs, guided by relevant literature and previous studies. Our scientific team is available to assist with experimental design, model selection, and data analysis, ensuring a tailored and effective approach for your project.
Related Services
The Kidney Transplantation Model offers comprehensive insights into transplant-related research and therapeutic evaluation. In addition to this, we provide a range of other relevant models, including those for kidney diseases, fibrosis, and nephropathy, to further enhance your research capabilities and support diverse therapeutic studies.
- Autoimmune Nephropathy Models
- Acute Kidney Injury (AKI) Models
- Chronic Kidney Disease (CKD) Models
- Metabolic Nephropathy Models
- Hyperuricemia Models
- Cystitis Models
Our advantages
- Expertise: We have years of experience in creating and optimizing preclinical models across various therapeutic areas.
- Custom Solutions: Tailored experimental design and model selection to meet your specific research goals.
- Comprehensive Services: From model development and drug efficacy testing to data interpretation and publication-ready results.
- High-Quality Data: We employ advanced technologies to ensure accurate and reliable results.
- Collaborative Approach: Our scientific team works closely with you at every stage of your project, ensuring smooth and efficient execution.
Work with Us
- Summarize the project requirements and fill in the information collection form.
- Sign a CDA from both parties to further communicate information, such as targets.
- Select an animal model, discuss experimental design, and determine assay parameters.
- Project costing and project schedule forecasting.
- We provide a detailed project plan, including the required sample quantities, methods, and protocols.
- Both parties confirm the project details and start the project.
- Confirm the timeline of the project.
- We provide periodic results and information on the animal's condition.
- We will work together to make project adjustments as necessary.
- We provide a comprehensive project report promptly.
- We arrange transportation for the produced samples.
- We provide a discussion of the project results and help to arrange the next steps.
- Data storage and archiving.
FAQs
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1. What types of kidney transplantation models do you offer?
We offer allogeneic, autologous, and syngeneic rodent models to replicate different aspects of human kidney transplantation, including graft survival, immune response, and transplant rejection.
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2. Can you customize a kidney transplantation model to meet specific research needs?
Yes, we can tailor our kidney transplantation models based on your specific experimental design and therapeutic focus. Our team works closely with you to optimize the model for your project.
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3. What parameters are assessed in kidney transplantation models?
We evaluate parameters such as graft function, immune cell infiltration, histological changes, serum biomarkers (e.g., creatinine, BUN), and cytokine profiles, among others.
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4. How do you ensure the quality of your kidney transplantation models?
Our models are developed and tested by experienced scientists using the latest techniques in the field. We adhere to strict quality control standards, ensuring that all data generated is accurate and reliable.
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5. Can your models be used to evaluate immunosuppressive drugs?
Yes, our kidney transplantation models are ideal for testing the efficacy of immunosuppressive agents, organ preservation techniques, and strategies to prevent transplant rejection.
Published Data
Fig.2 The histopathological characteristics of arterial walls on day 7 post-operationally.1
The objective of this study was to evaluate the feasibility of two suture techniques within a rat kidney transplantation model and compare their performance with the traditional suture technique. The study focused on assessing the impact of these techniques on the survival rate and renal function of transplanted kidneys following allogeneic kidney transplantation. Additionally, histopathological analysis of the arterial walls was performed on day 7 post-operation. (a) Hematoxylin-Eosin Staining: Representative images of the arterial wall, stained with hematoxylin-eosin, showed thickening of the connective tissue (indicated by black arrows), with visible residual sutures (green arrows) at the arterial anastomotic site. (b) Verhoeff’s Van Gieson Staining: The arterial walls were further analyzed using Verhoeff's van Gieson stain to highlight the elastic fiber layer (black wavy lines) and the collagen fiber layer (red) within the vascular wall. These features were visible across all groups. A small amount of thrombus was observed in the endothelial layer of the lumen, particularly in the I suture group, where blood vessels were stained. This study provides insights into the histopathological differences between the suture techniques and their potential impact on kidney transplantation outcomes.
Reference
- Lu, Lan-Tao et al. "Anterior-interrupted and posterior-continuous suture technique improved the success rate of kidney transplantation model in rats." Acta cirurgica brasileira vol. 39 e396024. 20 Sep. 2024. https://doi.org/10.1590/acb396024 Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.