Immunodeficient mice engrafted with human cells and tissues have provided a promising alternative to in vitro studies with human tissues for the study of human immunobiology. The immunodeficient mice can permit efficient engraftment with human hematopoietic cells and then generate a functional human immune system. These humanized mouse models are becoming increasingly important for preclinical studies of human immunity, tissue regeneration, hematopoiesis, cancer, and infectious diseases etc. One approach for generating humanized mice to investigate the human immune system is to engraft mature lymphoid cells, which is so-called the human peripheral blood lymphocyte SCID (Hu-PBL- SCID) model. Creative Biolabs is able to provide the validated humanized PBL SCID mice for our worldwide clients to evaluate the safety and efficacy of potential novel agents to modulate the immune system.
Humanized PBL SCID Mouse
The severe combined immunodeficiency (SCID) is a severe immunodeficiency genetic disorder that is characterized by the complete disability of the adaptive immune system to mount, coordinate, and sustain an appropriate immune response, generally due to the absence of T and B lymphocytes. Severe combined immunodeficiency (SClD) mice can be stably engrafted with human peripheral blood lymphocytes, generating humanized PBL SCID chimeras; basically, these are mice with a human immune system.
The Hu-PBL-SCID model is established simply by injection of human peripheral blood mononuclear cells or spleen or lymph node cells into immunodeficient mice. Recent modifications of the humanized PBL-SCID mouse models have allowed investigation of the infiltration and rejection of human islet, skin and arterial allografts and the cell populations related to the immune rejection process.
Establishment of Humanized PBL SCID Mouse
Fig. 1. Approaches for the engraftment of human immune systems in immunodeficient mice. To establish Hu-PBL-SCID (human peripheral blood lymphocyte (PBL)-engrafted severe combined immunodeficiency (SCID)) mice, PBLs are delivered to adult recipients via intravenous, intraperitoneal or intrasplenic injection. (Shultz et al., 2012)
Features
The experimentally advantageous features of the humanized PBL-SCID mice have made them particularly valuable in many areas of biomedical research. At Creative Biolabs, our experienced scientists have established a large array of humanized PBL SCID mouse models that are verified by advanced technology and these models are available for delivery or being incorporated into our drug efficacy testing services.
As a world leader in the industry of animal model, Creative Biolabs also offers other Humanized Mouse Models you may be interested in:
If you are interested in learning more about Creative Biolabs' animal models or drug discovery service, please contact us for more details.
Reference
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