Creative Biolabs has successfully applied Sleeping Beauty transposon-based transgenic technology in the generation of transgenic animal models with stability and high efficiency. We are willing to share this expertise with our customers globally to meet their specific research needs.
Sleeping Beauty (SB) transposon is a synthetic transposon of the Tc1-like mariner family. It was originally discovered in salmon, where it had been silenced through evolution and was then reconstructed by investigators based on molecular phylogenetic data to recover its ability to transpose the genome. The first functional version of SB transposon is SB10, which was so named because the reconstruction process required ten major steps. Thereafter, continued efforts have been made by researchers in order to improve its ability of gene transfer. As a result, a novel SB transposase, SB100X, which shows high efficiency of stable gene transfer comparable to stable transduction efficiencies of integrating viral vectors, was developed to greatly boost its research application.
The Sleeping Beauty System
The Sleeping Beauty transposon is a two-component system comprised of an SB transposase and a transposon. The transposase is the enzyme required for catalysis of transposition and the transposon contains an expression cassette of interest which is flanked by two inverted repeat/direct repeat (IR/DR) sequences. By specifically binding to these sequences, transposase mediates the excision and precise integration of the target cassette into the specific sites of the genome. Generally, the specific integration site for SB transposon is the TA dinucleotide base pair.
Fig.1 Sleeping Beauty transposon mutagenesis in
rat spermatogonial stem cells.
(Ivics et al. 2011) Features of Sleeping Beauty Transposon System
Compared to other methods of gene transfer such as the use of viral vectors and naked DNA, SB transposon exhibits great advantages. For viral vectors, although high efficiency can be obtained, they can be susceptible to cells' defense mechanisms. Besides, they are more efficient than plasmid-based methods. In summary, transposons combine the advantages of these two methods and avoid many of these problems.
When compared to other transposons, however, SB shows lower tolerance of cargo size, which means increased cargo size exponentially decreases the efficiency of transposition. As is reported, when the size of the payload between IR/DRs increases above 2 kb, the transposition efficiency drops dramatically. But still, SB transposon system is widely used in different fields and modified versions have been developed to expand its application.
Meanwhile, Creative Biolabs is capable of providing other model creation services to satisfy your various needs. Please click the links to find more useful information:
Besides Sleeping Beauty, other types of transposon-mediated transgenic services including PiggyBac and Tol2 transposons are also included in our list. At Creative Biolabs, having sufficient practical experience and skilled techniques, we totally understand how to help design the studies to obtain rapid and clear data to our clients. Here, you can find the most comprehensive model creation services at most favorable prices. Please feel free to contact us or send us an inquiry for more information or a formal quote.
Reference
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