Creative Biolabs focuses on cell scratch assay to meet the needs of customers for scientific research and related biomedical industries.
Cell migration is a hallmark of tumor development and is often associated with angiogenesis to ensure tumor nutrition, as well as metastasis formation. Developing methods to study migration is essential to study the mechanisms of tumorigenesis.
Cell scratch assay is a commonly used assay to test cell migration as it is economical and easy to perform on adherent cell lines such as endothelial cells, epithelial cells, and fibroblasts. Creative Biolabs offers in vitro cell scratch assay services to assist clients' projects in cancer research.
Fig.1 The model of cancer cell migration and invasion. (Novikov, 2021)
In cell scratch experiments, an adherent cell line that binds properly to the bottom of the well is generally required to detect and analyze migration. In order to measure migration, we usually modify the appropriate culture conditions in advance, such as reducing the serum concentration, starving the cells before scraping or using some inhibitors. Root according to the different cell types to choose the appropriate measures. After pre-culturing adherent cells for a period of time, uniform scratches will be carried out mechanically with pins in 96-well format. After the application of the test compound, the migration of incubated cells into the scratched area is detected by the IncuCyte instrument in real-time.
Fig.2 The scheme of the cell scratch assay process. (Creative Biolabs)
Result:
Fig.3 Migration of pre-treated mitomycin C HaCaT cell over 48 hours under sample addition. (Mouritzen, 2018)
Fig.4 Graphic illustration of migration under the treatments. (Mouritzen, 2018)
Q: Why choose cell scratch assay?
A: The cell scratch assay is a low-cost, easy, and well-developed experiment type to detect cell migration in vitro. In addition, the assay enables real-time monitoring of cell migration and does not require labeling.
Q: Which cell types does this assay apply to?
A: Adherent cell lines such as endothelial cells, epithelial cells, and fibroblasts.
For more details about our cell scratch assay services, please don't hesitate to contact us for more information.
References
For Research Use Only.
