Hi-Affi™ Monoamine Oxidase Assay Kit-Colorimetric (CAT#: ZL-0424-WR18)
| Overview | |
|---|---|
| Description | This kit is a simple and sensitive quantitative colorimetric assay for measuring amine oxidase activity in biological samples. |
| Features | Ready-used for oxidase/peroxidase activity assay. |
| Applications | Useful for end point and kinetic measurements of Monoamine Oxidase (MAO) activity, as well as semicarbazide-sensitive amine oxidase (SSAO). |
| Assay Measures | Colorimetric |
| Research Areas | Oxidative stress; Cell Damage; Drug Discovery |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | This kit includes a 96-well Protein Binding Plate, Colorimetric Probe, HRP, Hydrogen Peroxide, Tyramine, Benzylamine, MAO-A Inhibitor, MAO-B Inhibitor, and Assay Buffer. |
| Target/Description | Amidst the enzymatic reaction, Monoamine Oxidase acts on its substrate, producing hydrogen peroxide (H₂O₂). When coupled with HRP, the Colorimetric Probe interacts with the H₂O₂ to yield a distinct red/pink colored compound. This product can be conveniently measured using a microplate reader within the 540-570 nm range. The recorded absorbance values are directly connected to the levels of amine oxidase in the samples. The unknown sample quantities are discerned through a comparison with a hydrogen peroxide standard curve. |
| Format | Colorimetric assay kit. |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Ice bags; Avoid light |
| Regulatory Status | RUO |
For Research Use Only.
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