Based on the unrivaled expertise and experiences in antibody discovery and development, Creative Biolabs has developed a novel in vitro antibody-dependent enhancement assay service for global clients to speed up the project’s development.
Antibody-dependent enhancement (ADE), known as immune enhancement, involves a cascade of events whereby viruses may infect susceptible cells via interaction between virions complexed with antibodies (or complement components) and, respectively, Fc (or complement receptors), leading to the amplification of their replication increasing the infectivity and virulence.
ADE functions in viral infection with two distinct mechanisms: by enhancing antibody-mediated viral absorption into phagocytes expressing Fc gamma receptor IIa (FcγRIIa), leading to increased viral infection and replication, or by excessive antibody-mediated FC-mediated effector function or immune complex formation, causing enhanced inflammation and immunopathology.
Fig. 1 Antibody-dependent enhancement assay.1
During the past years, ADE has been reported for several viruses such as dengue virus, yellow fever virus, zika virus, and more importantly in the content of COVID-19, coronaviruses (CoVs), and so on. More importantly, ADE has been linked to the development of cytokine storm, which occurs in the most severe cases of MERS, SARS, and COVID-19 infection. Therefore, antibody-dependent enhancement (ADE) of disease is a particularly important consideration for the development of vaccines and antibody therapies. In vitro ADE assay is a valuable tool to assess the capacity of antibodies to enhance secondary infection with related viruses and provide valuable insights into the pathogenesis of virus infections in the development of vaccines.
Creative Biolabs is a world-leading services provider in the field of antibody discovery and development. Experienced in antibody function assays development, we are dedicated to supporting custom high-quality in vitro ADE assay service based on the immunostaining and flow cytometry analysis, greatly improving the accuracy and sensitivity of the detection. In addition, we are devoted to designing comprehensive research practices and handling high-quality studies to support your biopharmaceutical development pipeline.
Creative Biolabs’ scientists are dedicated to bringing together years of valuable experience to help our clients shorten the project journey. For more detailed information, please feel free to contact us or directly sent us an inquiry.
Reference
For Research Use Only.
