Ion Channel targeted Antibody Functional Characterization

As a pioneer and the undisputed global leader in antibody function analysis, Creative Biolabs is proud to offer our clients a series of Fab function analysis and characterization services with the best quality and most competitive price. Here, we are happy to introduce our professional service of GPCR-targeted functional assays with our diverse technologies.

Ion Channels as Drug Targets

Ion channels are a large family of membrane proteins that function by allowing ionic passage across cellular membranes. Present in all cell types, ion channels play critical roles in both physiology and disease by modulation of cellular functions such as electrical excitability, secretion, cell migration, and gene transcription. Mutations of single ion channel proteins have been demonstrated to be the cause of genetic diseases, collectively known as channelopathies. Due to the critical roles of ion channels in different biological processes, they represent an important class for therapeutics discovery. However, to date, small-molecule drugs, and peptide modulators dominate the ion channel-targeted drug discovery field. There exists a significant opportunity to target these channels with antibodies and other formats of biologics.

Fig.1 The mechanism of anti-hOrai1 channel monoclonal antibody (mAb) action on the human T-cell.^1,3

Advantages of Targeting Ion Channels with Antibodies

Antibodies display many advantages when compared to their small-molecule counterparts, including selectivity, bioavailability, and effector functions. Firstly, antibodies deliver superior specificity and selectivity targeting specific ion channel isoforms, thereby mitigating off-target effects and generating safer classes of drugs. Secondly, antibodies have limited central nervous system penetration and low variability in patient pharmacokinetics. Through different modifications, the half-life of antibodies can be extended to induce longer duration of action leading to reduced dosing. Thirdly, the potency and affinity of antibodies can be further enhanced and optimized by altering the Fc glycosylation status and using protein engineering methodologies.

Due to the above advantages, developing antibody-based therapeutics targeting ion channels can be of great importance for disease therapy. As a leading service provider of antibody development, Creative Biolabs offers professional anti-ion channel antibody discovery services via multiple state-of-the-art technologies.

Ion Channel-targeted Antibody Functional Characterization

Moreover, Creative Biolabs offers functional assays to help identify antibodies that modulate ion channel function. We use the accepted gold standard method for ion channel functional characterization, patch clamp electrophysiology, which allows real-time kinetic and pharmacological analysis of the effects of drug molecule candidates. We are capable of performing patch clamp assays using our cell lines that express specific ion channels. Particularly, we focus on a wide spectrum of ion channels that are implicated in human diseases, including but not limited to:

• Sodium channels
• Potassium channels
• Calcium channels
• TRP channels
• Kir channels
• Other ion channels

Featured Advantages

• Characterize the impact of antibodies on ion-channel activity through various advanced technologies.
• Collaborate with experienced scientists to design experiments specific to your research needs and the ion channel of interest.
• Utilize state-of-the-art equipment and rigorous data analysis methods to ensure high-quality and reliable results.

Data Display

This study highlights the potential of antibodies as targeted modulators of N-methyl-D-aspartate receptors (NMDARs). By identifying a functional antibody that specifically binds to the GluN1-GluN2B NMDAR subtype and allosterically inhibits its activity, as demonstrated through electrophysiological recordings, this research underscores the feasibility of using antibodies to selectively regulate distinct NMDAR functions. This approach has significant implications for both basic research, where these antibodies can serve as valuable tools for investigating NMDAR-mediated processes, and for therapeutic development, where they may offer a promising strategy for addressing neurological disorders and diseases associated with dysregulated NMDAR activity.

Fig.2 Isolation and characterization of specific ion channel-targeted IgGs.^2,3

Creative Biolabs has accumulated years of experience in both ion-channel targeted antibody development and functional characterization to discover therapeutic antibodies targeting specific ion channels. With combined technology platforms and know-how, we are confident in providing first-class services with fast turnaround time and competitive prices. For more detailed information, please feel free to contact us or directly send us an inquiry.

References

1. Haustrate, Aurélien, Aline Hantute-Ghesquier, and Natalia Prevarskaya. "Monoclonal antibodies targeting ion channels and their therapeutic potential." Frontiers in Pharmacology 10 (2019): 435761.
2. Tajima, Nami, et al. "Development and characterization of functional antibodies targeting NMDA receptors." Nature Communications 13.1 (2022): 923.
3. Distributed under Open Access License CC BY 4.0, without modification.

For Research Use Only.