Cyclophosphamide-Induced Cystitis (CYP) Model

Preclinical animal models not only contribute to recent advances in the pathogenesis understanding but also continue to provide the means of testing and evaluating new therapeutic agents for the treatment of cystitis. For the screening, testing, and evaluation of new drugs and formulations, Creative Biolabs offers several rodent models of cystitis, such as CYP model.

What is Chemically-Induced Cystitis?

Fig.1 Effect of chronic CYP treatment on mast cells
infiltration in mouse bladder tissue. (Golubeva et al. 2014).

Inflammation of the lower urinary tract is a relatively common type of inflammation that occurs frequently in people while the causes remain unclear. Animal models of lower urinary tract inflammation have proven to be useful for investigators to study the underlying mechanisms and options for suppressing relevant symptoms. Cystitis model induced by intravesical instillation of chemical irritants, including cyclophosphamide and acrolein, is one of the most commonly used experimental models to explore mechanisms underlying pain and inflammation associated with cystitis as well as to discover new remedies. The advantage of these models is that they allow control of the timing, duration, and severity of inflammation.

Cyclophosphamide (CYP)-Induced Urinary Bladder Inflammation

Cyclophosphamide (CYP)-induced urinary bladder inflammation is a well-established experimental model for bladder pain syndrome. CYP-induced cystitis model displays a local inflammation, associated with an increase in voiding frequency and a decrease in urine volume per void. Consequently, the CYP-induced cystitis model is widely used in rats and mice to characterize new inflammatory and nociceptive pathways, and identify new mediators. Conventionally, the chemical cystitis group was induced by a single intraperitoneal (i.p.) injection of CYP endotoxin, which is an antineoplastic agent that ultimately results in the formation of acrolein in the liver of patients treated with this drug, thus damaging the bladder urothelium and causing hemorrhagic cystitis as well as some other complications. Moreover, Creative Biolabs offers preclinical assessment services, which typically including but not limited to:

• Body Weight
• Bladder Weight
• Biomarker Analysis
• Histological Evaluation
• Cystometrogram Recording
• Pain Scoring

Meanwhile, Creative Biolabs also offers other types of rodent urological disease models that you may be interested in:

• Unilateral Ureter Obstruction (UUO) Model
• Thy-1 Nephritis Model
• Anti-Glomerular Basement Membrane (GBM) Nephritis Model
• 5/6 Nephrectomy Model
• Adriamycin-Induced Nephropathy (AN) Rodent Model
• Gentamicin-Induced Acute Renal Failure Model
• Cisplatin-Induced Acute Renal Failure Model
• Glycerol-Induced Acute Renal Failure Model
• Renal Ischemia-Reperfusion (IR) Model
• Lipopolysaccharide (LPS)-Induced Cystitis Model

As one of the leading nonclinical CROs for drug discovery and development, Creative Biolabs is capable of providing both validated rodent models and flexible, customized models. We help to choose the most clinically relevant and reliable models for testing your compounds and will keep a good communication with our clients during the on-going studies to get reliable results.

Reference

1. Golubeva, A. V.; et al. The mouse cyclophosphamide model of bladder pain syndrome: tissue characterization, immune profiling, and relationship to metabotropic glutamate receptors. Physiol Rep. 2014,2(3): e00260.

For Research Use Only.