Lipopolysaccharide (LPS)-Induced Cystitis Model

The LPS-induced cystitis models play an important role in investigating mechanisms underlying pain and inflammation associated with cystitis as well as exploring new strategies against pain and inflammation. With years of experience in the field of preclinical in vivo and in vitro studies, Creative Biolabs is capable of offering our customers the most comprehensive preclinical test system to meet every specific requirement precisely.

What is Interstitial Cystitis (IC)?

Interstitial cystitis (IC), also called bladder pain syndrome (BPS), is a type of chronic condition caused by inflammation of the bladder wall. The clinical symptoms of IC are often involved with a constant condition of recurrent urinary frequency, nocturia, suprapubic and pelvic pain. Currently, there is no cure for interstitial cystitis and the etiology remains unknown, although some theories exist as to the underlying mechanisms of this syndrome, and it is for this reason, together with the urgent need to develop new therapeutic approaches, that animal models of interstitial cystitis are being studied.

Fig.1 Histological examination of IC bladder and
quantification of mast cells. (Choi et al. 2014)

Overview of LPS-Induced Cystitis Models

Experimental cystitis models have been induced by intravesical instillation of a variety of irritants, among which bacterial lipopolysaccharides (LPS), a well-characterized biological modifier, is frequently and extensively used in the development of cystitis models and many other organ-specific inflammatory models. Lipopolysaccharides (LPS) are derived from components of the cell wall of gram-negative bacteria (e.g. E. coli) and can be used to create animal models of bladder inflammation via either intraperitoneal injection or intravesical instillation. The LPS infusion induces similar pathophysiology features observed in patients with interstitial cystitis. Creative Biolabs offers preclinical assessment services, which including but not limited to:

• Body Weight
• Bladder Weight
• Biomarker Analysis
• Histological Evaluation
• Cystometrogram Recording
• Pain Scoring

LPS-Induced Cystitis Model in Rats and Mice

In mice, cystitis can be initiated by instillation of E. coli LPS alone into the bladders, leading to the occurrence of symptoms such as edema, hemorrhage, and infiltration of neutrophils into the bladder wall.

In rats, the bladder injury is typically induced after first altering mucosal permeability with protamine sulfate, followed by intravesical instillation of Escherichia coli endotoxin lipopolysaccharide. Besides, intraperitoneal and intravenous injection of E. coli LPS could also stimulate inflammation in rats and mice, characterized primarily by edema within the bladder wall.

Meanwhile, Creative Biolabs also offers other types of rodent urological disease models that you may be interested in:

• Unilateral Ureter Obstruction (UUO) Model
• Thy-1 Nephritis Model
• Anti-Glomerular Basement Membrane (GBM) Nephritis Model
• 5/6 Nephrectomy Model
• Adriamycin-Induced Nephropathy (AN) Rodent Model
• Gentamicin-Induced Acute Renal Failure Model
• Cisplatin-Induced Acute Renal Failure Model
• Glycerol-Induced Acute Renal Failure Model
• Renal Ischemia-Reperfusion (IR) Model
• Cyclophosphamide-Induced Cystitis (CYP) Model

Creative Biolabs is dedicated to the development, production, and distribution of the best and most appropriate rodent disease models for our customers. We offer not only LPS-induced cystitis rodent models but also CYP-induced models and other customized cystitis models. Please feel free to inquire us or contact us directly if you are interested in any of our services.

Reference

1. Choi, B.H.; et al. Mast cell activation and response to tolterodine in the rat urinary bladder in a chronic model of intravesical protamine sulfate and bacterial endotoxin-induced cystitis. Molecular Medicine Reports. 2014,10(2):670.

For Research Use Only.