Acute or chronic myocardial infarction are cardiovascular events resulting in high morbidity and mortality. As a technology leader CRO in the preclinical drug development, Creative Biolabs provides preclinical evaluation of agents that are useful for the treatment of myocardial infarction in both acute and chronic rodent models of myocardial infarction.
Introduction of Myocardial Infarction
Myocardial infarction, commonly known as a heart attack, occurs when myocardial ischemia, a diminished blood supply to the heart, exceeds a critical threshold and overwhelms myocardial cellular repair mechanisms designed to maintain normal operating function and homeostasis. Ischemia at this critical threshold level for an extended period results in irreversible myocardial cell damage or death. Research models of infarction and myocardial ischemia are essential to investigate the pathobiological and pathophysiological processes in MI and to develop and optimize the future treatment.
Fig.1 Pictorial representation of myocardial ischemia. (Kumar et al. 2016)
Available Rodent Myocardial Infarction Models at Creative Biolabs
Rodent animals are frequently used lab animals for cardiovascular research because of their small size, relatively inexpensive cost, ease of handling and maintenance. Creative Biolabs conducts contract efficacy studies in different myocardial infarction models, including surgical ligation models, chemically induced models, and genetically modified mice models. Different models should be chosen based on the specific research question. With years of experience in myocardial infarction studies, we will work together with our clients in providing guidance of model choice, customized study design, end-point analysis, and help in data interpretation. The following acute and chronic myocardial infarction rodent models are available:
Ischemia-Reperfusion – It is an acute model of myocardial infarction, which involves surgical dissection into the chest cavity to expose the left anterior descending artery (LAD) that is then occluded by a ligature for a defined period in time to produce the ischemic event. This model is widely used for the study of infarct size limitation, and thus is used to develop innovative treatments to reduce the size of myocardial infarction. It can be induced in both rats and mice. Moreover, we utilize a modified surgical method to increase the animal survival rate and to achieve uniform infarction size.
Myocardial Infarction – It is induced by permanent ligation of the LAD without reperfusion. This model is suited for post-infarction remodeling and failure, and for the investigation of scar tissue and inflammation related to tissue repair. It can be modeled in both rats and mice.
Isoproterenol-Induced Myocardial Infarction – Isoproterenol is a chemical widely used to induce acute myocardial infarction in rats and mice. This model offers several advantages over several advantages over the surgical models, such as low mortality rate, simplicity, and no chance of post-surgical infections.
Genetically Modified Mice – With the genetically engineering platform, Creative Biolabs is able to conduct efficacy studies in different genetically modified mice models of myocardial infarction, such as LDLR -/-, ApoE -/- mice, and other transgenic mice.
Assessments for Drug Evaluation
To evaluate the cardioprotective effects and effects on ventricular remodeling and dysfunction of novel drugs, Creative Biolabs offers a wide range of end-point analysis with different techniques. These assessments include but are not limited to:
Additionally, Creative Biolabs offers contract efficacy studies in the following rodent cardiovascular models:
Creative Biolabs provides services with the advantage of customized experimental designs, expert scientific guidance, and consultancy. Moreover, we are capable of developing new models and assays to fulfill our clients’ needs. Contact us to discuss your project and experience the great value of our services.
Reference
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