Chronic kidney disease (CKD) is defined as the progressive loss of kidney function over a period of months or years, which is a worldwide public health problem with rising incidence and prevalence. Creative Biolabs offers various chronic kidney disease models in rats and mice for preclinical screening, testing, and evaluation of new drugs and formulations.
Complete Unilateral Ureter Obstruction (UUO)
Although complete ureteral obstruction is not one of the most common causes of human renal disease, UUO has become one of the most popular models of chronic kidney disease due to its capability of generating a progressive renal fibrosis, thus making it useful to examine mechanisms of tubulointerstitial fibrosis in vivo as well as a good model for screening new therapies. The complete obstruction model has the advantages of good reproducibility (hence inter-animal variation is not a problem), a short time-course, easy performance, and the presence of the contralateral kidney as a control. Besides, this model can be easily induced in both rats and mice. The major disadvantage is that only modest changes occur in such markers as serum creatinine and urea concentrations.
Complete UUO initiates a rapid sequence of events in the obstructed kidney, leading within 24 h to reduce renal blood flow and glomerular filtration rate. The subsequent interstitial responses include interstitial inflammation, tubular dilation, renal tubular apoptosis leading to tubular atrophy and fibrosis from 7 days. The obstructed kidney reaches end stage by around 2 weeks.
Partial and Reversible UUO
Variations of this model have been developed including the obstruction of kidneys in neonatal rats and mice and the relief of obstruction after a few days, which are called partial and reversible UUO models, respectively. Models of partial UUO are developed because most cases of clinical congenital obstructive nephropathy involve partial instead of complete obstruction. Besides, these models are also useful in the study of the pathogenesis of renal fibrosis, because the lesions develop more slowly and may be better suited to the study of therapeutic manipulations. The latter model has proved a particularly useful model to study regeneration but the period of obstruction is inversely related to the capacity for repair.
Measurements provided by Creative Biolabs including but not limited to:
Fig.1 Interstitial of monocytes - macrophages after unilateral ureteric obs accumulation truction (UUO).
(Hewitson et al. 2009)
Meanwhile, Creative Biolabs also offers other types of rodent urological disease models that you may be interested in:
Particularly, our scientific team has extensive experience helping clients with the selection of appropriate models and assays, as well as collaborating to design new models to meet the specific needs of a particular compound or discovery program. Moreover, in addition to the validated models described above, we offer customized solutions by working with our clients to develop novel models for disease areas that may not be readily or easily standardized in the field.
For more details, please feel free to contact us to discuss your requirements and experience the value of our services.
Reference
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