Fluorescence polarization anisotropy (FPA) is the phenomenon that the light emitted by a fluorophore has unequal intensities along different axes of polarization. Moreover, it is a measurement of the changing orientation of a molecule in space, and about the time between the absorption and emission events.
Creative Biolabs is the company that holds a world-leading position in the development and characterization of therapeutic antibodies. Based on our advanced techniques, we can offer several services of FPA to help you to analyze, observe and get your desired drug candidates. Here, we introduce a wide range of technologies relies on FPA, such as Analytical Ultracentrifugation (AUC), Biolayer Interferometry (BLI), Surface Plasmon Resonance (SPR), Thermal Shift Assay (TSA), Isothermal Titration Calorimetry (ITC), Differential Scanning Calorimetry (DSC), and Dual-Polarization Interferometry (DPI).
Introduction to Fluorescence Polarization Anisotropy (FPA)
Measurements of FPA is a useful technique in biochemical research and medical testing. Fluorescence methods are widely used for the characterization of molecular interactions. Specially, polarization/anisotropy studies are widely used among the life sciences because they make quantification of protein interactions in the micro- and nanomolar concentration range.
Based on excitation with polarized light, the emission from many samples is also polarized. The extent of polarization of the emission is described in terms of the anisotropy (r), shown in Fig.1. Within this formula, I means fluorescent intensity; V for vertical and H for horizontal, mean the polarization direction of the two polarizers on the excitation and emission light path, respectively; and the ratio of IHV/IHH means calibrate for the difference in the emission channel’s sensitivity towards vertical and horizontal polarized components. Samples exhibiting nonzero anisotropies are said to display polarized emission. The origin of anisotropy is the existence of transition moments for absorption and emission that lie along specific directions among the fluorophore structure.
Fig.1 The measurement of the anisotropy (r). (Shi, 2009)
Applications of FPA in Drug Discovery
Optical techniques utilizing fluorescently labeled molecules have become dominant in drug discovery, spanning from molecular assays to full body imaging, and almost completely replacing the radio-labeling. FPA as a kind of measurement to observe and measure dynamic structural changes of macromolecules undergoing biochemical reactions. Based on FPA, scientists and researchers can employ this technology to check the interactions between decided protein targets and fragments, which is a crucial step in the drug discovery. Also screening appropriate drug candidates or any other protein interactions researchers required.
At the same time, FPA measurements are available methods for compounds characterization and to detect fragments or compounds binding for a series of essential clinical targets. Except that, it provides a basic principle to measure the structure of a protein in one dimension (i.e., its diameter or size) and the density (i.e., its mass per unit volume or how tightly folded it is). All these applications are useful and necessary during the projections of the drug discovery and development for observing and evaluating the drug potential candidates.
Services Relied on FPA in Creative Biolabs
Features of our Services
At Creative Biolabs, we provide high-quality services for customers to analyze the drugs candidates based on our advanced analytical techniques and years of experience. During the projects, we will communicate with clients for their project plans in time and design the suitable workflow based on their requirements. There are many advanced analytical methods, such as AUC, BLI, SPR, TSA, ITC, DSC, and DPI. If you want to know more information about FPA and other analytical techniques, please don’t hesitate to contact us.
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