Biolayer Interferometry (BLI)

Based on the advanced biolayer interferometry (BLI) technology platform established for years and experts experienced in the drug discovery, Creative Biolabs can provide incomparable BLI services to meet all your demands.

BLI technology refers to determining the biomolecular mutual effect. This technique is an optical analytic approach analyzing the interference pattern of white light reverberated from two surfaces: an inner reference layer, and a layer of settled protein on the biosensor tip. A variation in the interference pattern is induced by any altering in the number of molecules combined the biosensor tip. In this case, interactions can be gauged in real time, offering the capacity to supervise binding specificity, the ratio of association and dissociation, concentration, with fidelity and accuracy. In addition, only molecules combining with or separating from the biosensor are capable of changing the interference pattern and produce a reaction profile on the BLI system. However, molecules without binding, vary in the refractive index of the ambient medium or vary in flow rate have no effect on the interference pattern.

Figure 1. BioLayer Interferometry (BLI) technology assay principle. (Concepcion 2009)

Fragment-Based Screening

BLI is a useful tool for fragment screening in the drug discovery process. The biosensor-based fragment screening detects the interaction of a protein target settled on a biosensor with a fragment and kinetic constants are gained from binding data. Compared to other methods, BLI has a significant advantage that it can largely decrease false-positive rates because non-specific interactions can be distinguished from desired binding profiles and responses. Creative Biolabs can offer high-quality BLI services to determine the interactions between decided protein targets and fragments, which is a crucial step in the drug discovery.

Proteins-Ligand Interactions

BLI is a label-free method extensively used for the analysis and characterization of protein interactions. The determination of protein interactions allows researches further understanding the possible mechanism of drugs and gives an impetus to the drug discovery development. Based on professional knowledge of drug discovery and years of experience, Creative Biolabs can perform unbeatable protein interactions services using BLI technology to screen appropriate drug candidates or any other protein interactions you required.

Advantages of Biolayer Interferometry (BLI)

Kinetic characterization with the BLI enables to replace or complement label-dependent methods. Label-free, real-time analysis of BLI can offer quickly, accurate, and sensitive determination of kinetics, affinity and activity of complex formation. Using BLI technique, it's available to get detailed information about the mechanism of interaction between molecules. What's more, BLI is not only simple and fast to implement, importantly, it also has crude sample compatibility.

Creative Biolabs provides other various drug discovery services. For more detailed information, please feel free to contact us or directly sent us an inquiry.

Reference

1. Concepcion J (2009). “Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization”. Combinatorial chemistry & high throughput screening 12(8), 791-800.
2. Wallner J (2013). “Application of Bio-Layer Interferometry for the analysis of protein/liposome interactions”. Journal of pharmaceutical and biomedical analysis 72, 150-154.
3. Sultana A (2015). “Measuring Protein‐Protein and Protein‐Nucleic Acid Interactions by Biolayer Interferometry”. Current Protocols in Protein Science 19-25.

For Research Use Only.