Multi Angle/Dynamic Light Scattering (MALS)

Multi angle/dynamic light scattering (MALS) is a non-invasive, fast technique for determining both the absolute molar mass and the average size of molecules in solution. Based on this technique, Creative Biolabs offers the best hit characterization services involved in determining size, concentration, conformation molecular weight of compounds to ensure active and stabile compounds for further screening, accelerating the process of drug discovery.

Introduction of MALS

MALS is a well-established technique for measuring the size and size distribution of molecules and particles dispersed in a liquid. The principle of this technique is to analyze the temporal fluctuations of light scattered by the particles at a given scattering angle and the particle size is calculated by using the Stokes-Einstein relationship. Compared to single-angle dynamic light scattering, MALS provides more robust, reproducible and accurate particle size distributions. This technique is also used to directly measure molar mass of macromolecules and complexes in solution. By utilizing MALS in conjunction with size exclusion chromatography (SEC) and an on-line concentration detector, the absolute molar mass and size of biological macromolecules can be determined. Furthermore, composition-gradient MALS (CG-MALS) uses a composition-gradient system coupled to MALS/RI/UV detectors to determine interactions between biological macromolecules including protein-protein or protein-DNA complexes, without the need for labeling or immobilization, by directly detect changes of molar mass due to complex formation or dissociation in solution.

Fig.1 Dynamic light scattering.

Our Services

With cutting-edge technique and professional expert staff, we have gained extensive experience in biophysical characterization of drug candidates. Based on this powerful technique, we offer:

• Measuring the size and size distribution of molecules and particles in suspension
• Measurements of particle concentration
• Determining absolute molecular mass of macromolecules and complexes
• Determining the binding affinity and stoichiometry of interacting molecules
• Monitoring the presence and formation of aggregates

Advantages

• Easy-to-prepare samples
• Low sample volume requirement as little as 3 µL
• Accurate, repeatable and reliable particle size and concentration analysis
• Measurement range of particle size: 0.5 nm to 10 um
• Measurement range of molecular weight: 1000 Da to 1000 MDa

Creative Biolabs is an outstanding expert in the field of drug discovery who provides a full range of services with exceptional quality and reliability and low cost to promote drug discovery. Equipped with the MALS technique, we support the measurement of size, molecular weight, concentration, and molecular conformation of active compounds as well as determining their binding affinity to interacting molecules.

If you are interested in our services, please feel free to contact us for more details and a customized quote.

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