Hi-Affi™ pH-Sensitive Dye, Epidermal Growth Factor (EGF) Conjugate-Green (CAT#: DR-LX054)

Datasheet
Overview
DescriptionCreative Biolabs offers a wide range of dye reagents and kits suitable for evaluating cell biological activity such as apoptosis, endocytosis and can be used in a number of areas: drug discovery, toxicity testing and immunology. Hi-Affi™ pH-Sensitive Dye, Epidermal Growth Factor (EGF) Conjugate-Green is an ideal tool for the detection of internalized EGF receptor in cells.
FeaturesConvenient, and simple steps;
Be compatible with various antibody function study platforms;
Reduced signal variability and improved timing in sensitive experiments;
ApplicationsProduct is suitable for various application platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometry, and high throughput screening (HTS).
Molecular Mechanism of ActionHi-Affi™ pH-Sensitive Dye, Epidermal Growth Factor (EGF) Conjugate-Green is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes.
Assay MeasuresFluorescence
Research AreasDrug Discovery and Toxicity Testing; Immunology
Excitation509
Emission533
Specification
Size1 mg
StorageStore protected from light at -20 °C.
HandlingPlease place the product at proper storage condition once received.
ShippingIce packs

For Research Use Only.

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