Hi-Affi™ Intracellular Nitric Oxide (NO) Assay Kit-Fluorometric (CAT#: ZL-0424-WR21)
| Overview | |
|---|---|
| Description | Creative Biolabs' Hi-Affi™ Intracellular Nitric Oxide (NO) Assay Kit-Fluorometric offers a rapid and convenient method to measure nitric oxide (NO) or nitric oxide synthase (NOS) activity within cultured cells. |
| Features | Ready-used for ROS assay. |
| Applications | Efficient for the measurement of intracellular NO or NOS activity. |
| Assay Measures | Fluorometric |
| Research Areas | Oxidative stress; Cell Damage; Drug Discovery |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | This kit includes NOS Inhibitor (L-NNA), Cell Lysis Buffer, and Nitric Oxide Fluorometric Probe. |
| Target/Description | Following passive diffusion into the cells, cellular esterases deacetylate the cell-permeant NO probe to a non-fluorescent intermediate.When intracellular nitric oxide comes into contact with this intermediate, it quickly oxidizes to a highly fluorescent triazolofluorescein analog.Fluorescence intensity is proportional to the NO levels inside the cell's cytoplasm. |
| Format | Fluorometric assay kit. |
| Storage | Store at -20°C. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Ice bags; Avoid light |
| Regulatory Status | RUO |
For Research Use Only.
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