Hi-Affi™ Intracellular Nitric Oxide (NO) Assay Kit-Fluorometric (CAT#: ZL-0424-WR21)

Datasheet
Overview
DescriptionCreative Biolabs' Hi-Affi™ Intracellular Nitric Oxide (NO) Assay Kit-Fluorometric offers a rapid and convenient method to measure nitric oxide (NO) or nitric oxide synthase (NOS) activity within cultured cells.
FeaturesReady-used for ROS assay.
ApplicationsEfficient for the measurement of intracellular NO or NOS activity.
Assay MeasuresFluorometric
Research AreasOxidative stress; Cell Damage; Drug Discovery
Specification
Size96 tests
ComponentsThis kit includes NOS Inhibitor (L-NNA), Cell Lysis Buffer, and Nitric Oxide Fluorometric Probe.
Target/DescriptionFollowing passive diffusion into the cells, cellular esterases deacetylate the cell-permeant NO probe to a non-fluorescent intermediate.When intracellular nitric oxide comes into contact with this intermediate, it quickly oxidizes to a highly fluorescent triazolofluorescein analog.Fluorescence intensity is proportional to the NO levels inside the cell's cytoplasm.
FormatFluorometric assay kit.
StorageStore at -20°C.
HandlingPlease handle the product at appropriate storage condition once received.
ShippingIce bags; Avoid light
Regulatory StatusRUO

For Research Use Only.

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