PathSpecific™ Human XIAP Intrachain Assay Kit, TR-FRET (CAT#: ZL-0124-WR34)
| Overview | |
|---|---|
| Description | The kit is designed to measure the activity of XIAP, an inhibitor of apoptosis protein, in a homogeneous 384 reaction format. It allows for the quantitative assessment of XIAP auto-ubiquitination. |
| Features | Ready-used for measuring XIAP auto-ubiquitination activity. |
| Applications | Kit is used to screen compound and study to measure compound binding to the target. |
| Application Notes | Excellent for screening small molecule inhibitors in pharmaceutical research and high-throughput screening. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 384 tests |
| Components | The kit is supplied with an assay kit including UBE1, UbcH5b, XIAP, TRF Ubiquitin Mix (200x), ATP (4 mM), and U2 Assay Buffer. |
| Target | XIAP |
| Target/Description | The assay utilizes a Europium cryptate-labeled Ubiquitin (donor) and Cy5-labeled Ubiquitin (acceptor) to complete the TR-FRET pairing. This FRET-based assay eliminates the need for washing steps, making it suitable for high-throughput screening (HTS) and real-time kinetics analyses of polyubiquitination. The assay measures the fluorescence intensity, which is proportional to the extent of polyubiquitination on the target protein XIAP. |
| Format | Supplied with a TR-FRET assay kit. |
| Storage | Store at -80°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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