| Overview | |
|---|---|
| Description | The kit is used to quantify the activity of cathepsin S in cell lysates and tissue extracts. |
| Features | Ready-used for protease assay |
| Applications | Kit is used to study the Cathepsin S protease in biological samples. |
| Application Notes | The kit is a fluorescence-based assay that uses the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin) |
| Molecular Mechanism of Action | Cell lysates or other samples containing cathepsin S will hydrolyze the synthetic substrate VVR-AFC, releasing the fluorochrome AFC. The emitted fluorescence can be easily measured using a fluorometer or a fluorescence plate reader. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including CS cell lysis buffer, CS inhibitor (1 mM), CS reaction buffer, and CS substrate Ac-VVR-AFC (10 mM). |
| Target | Cathepsin S |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
