Bacterial Cytological Profiling Platform

Creative Biolabs has been a long-term expert in the field of antibacterial drug discovery. Equipped with world-leading technology platforms and professional scientific staff, Creative Biolabs is always dedicated to assisting our clients with the most satisfactory drug discovery related services.

Introduction of Bacterial Cytological Profiling (BCP)

The antibacterial field, especially the emergence of drug-resistant bacteria, provides a broad space for the development of antibacterial drugs. The common approach to identify antibacterial compounds is to screen inhibitors against bacteria, phenotypic screening, in vitro screening, or structure-based drug design. However, these methods require large amounts of bacteria, candidate drugs, and known control drugs. More importantly, the mechanism of action of candidate antibacterial agents cannot be understood in depth.

The identification of the mechanism of action for antibacterial compounds is essential in antibiotic discovery, but its complexity largely limits the development of both basic research and antibacterial discovery programs. Bacterial cytological profiling (BCP) is a rapid and powerful approach to identify the cellular pathway affected by antibacterial molecules. As a one-step assay, BCP presents great potentials to determine the cellular target of thousands of compounds rapidly and determine specific antibacterial mechanisms of action. Compared with traditional methods, BCP can be applied broadly to various species and antibiotics and can flexibly replace the current rapid drug susceptibility test method.

Fig.1 A 3D PCA graph for Spirohexenolide A MOA determination by BCP. (Nonejuie, 2013)

BCP-Based Services:

• Library screening for novel antibiotics identification.

We can screen both chemical libraries and natural product libraries, the preliminary activity and MOA information of the hits can be obtained easily.

• MOA Determination

For the antibacterial compounds, the MOA can be rapidly determined against both Gram-positive and Gram-negative bacteria. In addition, the subcellular binding location can be obtained.

• Identification of bactericidal effect

Using high-resolution microscopy, the images of antimicrobials-treated bacterial pathogens can be obtained. We can also determine how compounds enter bacterial cells.

Fig.2 Bacterial cytological profiling high-throughput screening (BCP-HTS). (Nonejuie, 2013)

Advantages of BCP Platform:

• Efficient to provide higher resolution for identifying various pathways.
• Can be performed in microliter scale culture volumes.
• Few specialized strains are required.
• Simple workflow allows high-throughput tests.
• Growth inhibition data, such as MIC or viable cell counts, are not required.

In response to the current severe situation for microbial diseases and emerging drug-resistant, Creative Biolabs is dedicated to the discovery and development of novel technologies that accelerate drug discovery. If you are interested in our services, please do not hesitate to contact us for more details.

Reference

1. Nonejuie, P.; et al. Bacterial cytological profiling rapidly identifies the cellular pathways targeted by antibacterial molecules. Proceedings of the National Academy of Sciences. 2013, 110(40): 16169-16174.

For Research Use Only.