PathSpecific™ Chymotrypsin Assay Kit, Fluorometric (CAT#: ZL-1122-WR22)
| Overview | |
|---|---|
| Description | The kit is used to quantify chymotrypsin activity in cell and tissue lysates. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the chymotrypsin protease in biological samples. |
| Application Notes | The kit is a fluorescence-based assay that use a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. |
| Molecular Mechanism of Action | Upon application of the chymotrypsin activator, chymotrypsinogen is converted into active chymotrypsin. This active form then breaks down the non-fluorescent substrate to release a stable fluorophore. This process can be easily tracked and quantified. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including chymotrypsin assay buffer, chymotrypsin substrate, chymotrypsin activator, chymotrypsin inhibitor, coumarin standard (1 mM), and chymotrypsin positive control. |
| Target | Chymotrypsin |
| Species Reactivity | Mammals, Other species |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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