PathSpecific™ Mouse/Human KIT(Phospho-Tyr703) Cell-Based Phosphorylation ELISA Kit (CAT#: ZL-0524-WR52)
| Overview | |
|---|---|
| Description | This kit is a convenient, lysate-free, high throughput, and sensitive assay kit that can monitor KIT protein phosphorylation and expression profile in cells. |
| Features | Ready-used for phosphorylation assay. |
| Applications | Useful for measuring the relative amounts of phosphorylated KIT in cultured cells as well as screening for the effects that various treatments, inhibitors, or activators have on KIT phosphorylation. |
| Assay Measures | Colorimetric |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 1 EA |
| Components | This kit includes User Manual, 96-well Cell Culture Microplate, Blocking Buffer, Detection Antibodies, HRP-Conjugate, Substrate, Stop Solution, Crystal Violet Solution, SDS Solution, and Adhesive Plate Sealers. |
| Target | KIT(Phospho-Tyr703) |
| Species Reactivity | Mouse, Human |
| Target/Description | Qualitative determination of KIT(Phospho-Tyr703) concentration is accomplished using an indirect ELISA format. KIT(Phospho-Tyr703) is captured by specific primary antibodies, and HRP-conjugated secondary antibodies bind to the Fc region of the primary antibody. This binding facilitates a colorimetric reaction catalyzed by the HRP enzyme upon substrate addition. |
| Format | Colorimetric Assay Kit |
| Storage | Store at 4°C. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Ice bags |
| Regulatory Status | RUO |
For Research Use Only.
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