Creative Biolabs has been a long-term expert in the field of antibacterial and antifungal drug discovery. Based on our extensive experience and world-leading technology platforms, Creative Biolabs is always dedicated to assisting our clients with the most satisfactory neutral bacterial protease assay.
Bacterial proteases are a broad collection of enzymes that play important roles in cell viability, stress response, and pathogenicity. In response to the worsening antibiotics crisis, the bacterial proteases perturbation presents great potential for novel antimicrobial drug discovery. A series of bacterial proteases offer an arsenal of novel antibiotic targets ripe, such as the signal peptidases (SPs) and caseinolytic protease (ClpP). In addition, the mild proteolysis of neutral proteases makes it particularly suitable for the preparation of primary cells and secondary cell cultures. It can also be used as an auxiliary enzyme in cell separation and tissue dissociation applications.
The bacterial neutral protease produced by bacillus is the most commonly used neutral protease on the market. Its enzymatic activity mainly depends on divalent cations, such as Mg2+, Zn2+, Ca2+.
The bacterial protease units can be defined as the quantity of enzyme that produces the equivalent of 1.5 μg/mL of L-tyrosine per minute at pH 7.0 and 37°C. The assay is based on the proteolysis of casein, where unhydrolyzed casein is removed by filtration and the dissolved casein is measured spectrophotometrically. In this assay, the substrate blank absorbance would be served as a reference to reflect the integrity of the assay and evaluate the reagent variability. After three replicates, the difference in results should be limited to 3%.
1. Enzyme preparation.
2. Enzyme evaluation.
3. Preparation of standard curve.
4. Calculation of PC.
Equipped with world-leading technology platforms and professional scientific staff, we are pleased to offer the most qualified services and products for antibacterial and antifungal drug discovery. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.
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