Creative Biolabs has been a long-term expert in the field of antibacterial and antifungal drug discovery. Based on our extensive experience and world-leading technology platforms, Creative Biolabs is always dedicated to assisting our clients with the most satisfactory serum bactericidal activity (SBA) assay.
As lethal antimicrobial activity is always required for the treatment of deep-seated infections, the novel serum bactericidal activity (SBA) assay has been developed as a comprehensive indicator of antimicrobial pharmacodynamics and pharmacokinetics. This test refers to the maximum dilution factor that kills more than 99.9% of pathogenic microorganisms within 18 hours after collecting the patient's venous serum and diluting the serum when the patient is treated with antibiotics. The SBA value can be obtained by C/MBC, where C represents the antibacterial drug concentration in serum and MBC is the minimum bactericidal concentration. In clinical practice, the peak value of SBA ≥ 1:8 and the trough value ≥ 1:2 are often regarded as the effective and reasonable range of antibacterial therapy. Compared with drug sensitivity test and pharmacokinetic test, the SBA assay can more accurately reflect the therapeutic effect of antibacterial drugs in vivo, so that presents important clinical application value.
To perform a typical SBA assay, serial dilutions of sera are incubated with target bacterial strains. The SBA reaction mixture will be plated on agar and surviving bacterial colony-forming units (CFU) will be counted at each serum dilution. To further improve the experimental efficiency, a luminescence-based SBA (L-SBA) method has been developed to detect surviving bacteria by measuring their ATP. Considering the susceptibility of the pathogen, our assay allows the measurement of the antibiotic absorption and elimination, the binding of the drug to serum proteins, the effect of the parent compound and metabolites against the infecting organism, as well as the effect of drug interactions.
Fig.1 Serum bactericidal assay with human complement. (Donald, 2017)
Equipped with world-leading technology platforms and professional scientific staff, we are pleased to offer the most qualified services and products for antibacterial and antifungal drug discovery. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.
Reference
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