Amino acids are very crucial organic compounds containing amine (-NH2) and carboxylic acid (-COOH) functional groups, along with a side-chain (R group) responding to each amino acid. They are a chemically diverse set of compounds present in proteins and peptides. Amino acids play critical roles in biological processes, such as biosynthesis neuro and transmitter transport. Amino acid composition analysis is one of the classical protein analysis methods which can be utilized to determine the composition and content of amino acids in protein/peptide, and non-typical amino acids in protein/peptide drugs.
Based on amino acid derivative technology and high-efficiency liquid chromatography, Creative Biolabs provides efficient and accurate amino acid composition analysis services for biopharmaceutical products, including single sequence analysis, multiple sequence analysis, as well as other analytic techniques to support the drug discovery and development.
Importance of Amino Acid Composition Analysis
The amino acid composition, shown in Fig.1, provides additional evidence on the assembly success for a given peptide. To confirm the results of peptide sequencing, it is possible to perform a quantitative amino acid analysis. It also provides quantitative information on amino acid ratios within a peptide and yields exact peptide content for accurate calculation of the peptide concentration. At the same time, the composition analysis of amino acids is a necessary step before the complete sequencing of biopharmaceuticals, and provides valuable evidence for the analysis of the sequence of biopharmaceuticals.
Fig.1 Amino acid composition. (Gromiha, 2005)
Amino Acid Composition Analysis Service
The service mainly consists of two steps:
Creative Biolabs uses phenyl isothiocyanate (PITC) for amino acid derivation, followed by a high-efficiency liquid chromatography for determining the composition and content of amino acid derivatives, so as to speculate the components of the amino acids in the protein/peptide samples.
Amino acid analysis is useful and crucial for a variety of applications in many different eras, such as:
Features of Our Services
The amino acid analysis services are important and necessary steps during the drug discovery and development processes, such as analyzing and observing the drug metabolism and functions of proteins. Therefore, Creative Biolabs offers amino acid composition analysis service for customers all over the world relied on advanced technological technologies and experienced researchers. Besides that, we also design and operate experiments based on customers’ requirements with affordable prices and a time save manner. If you are interested in our amino acid composition analysis service, please feel free to contact us.
Reference
For Research Use Only.
