Creative Biolabs has successfully established a range of experimental models of kidney diseases and in particular, we provide models that mimic human mesangial proliferative glomerulonephritis (Thy-1 Nephritis model) and anti-GBM nephritis (anti-GBM disease model). We are proud to offer the most validated and reliable models for testing your compounds and guarantee that any data collected is both biologically and clinically reliable and relevant.
Anti-Glomerular Basement Membrane (GBM) Nephritis Model
Human glomerulonephritis (GN) is a progressive inflammation of the glomeruli that can be caused by a variety of underlying disorders. As one of the most severe types of GN, anti-GBM nephritis is an autoimmune disorder in which circulating antibodies against type IV collagen bind to renal GBM and initiate an inflammatory reaction, which is similar to that seen in Goodpasture's syndrome. Patients with anti-GBM nephritis usually present with rapidly progressive glomerulonephritis, hematuria, and sub-nephrotic range proteinuria. Insight into the pathogenesis of human glomerulonephritis has been greatly enhanced by studies in experimental models, among which the model of anti-GBM nephritis is one of the oldest and most extensively studied.
Induction of Anti-GBM Nephritis Model
Murine models mimicking human anti-GBM nephritis require immunization with collagen type IV or heterologous anti-GBM antibodies which initiate an autoimmune antibody-mediated inflammatory response. Briefly, mice were pre-sensitized with rabbit IgG, given intraperitoneally (i.p.) in complete Freund's adjuvant. Five days later, the mice received a tail injection with rabbit anti-GBM serum administered intravenously (i.v.). Within 2 to 3 weeks, mice will exhibit pronounced glomerulonephritis with the crescent formation, as well as tubulointerstitial disease. Further, they will develop severe proteinuria and azotemia. Anti-GBM nephritis can be induced in both mice and rats, but great variation in susceptibility among strains exists. Generally, the Wistar Kyoto (WKY) rat and mice strains such as BUB/BnJ, DBA/1J, and 129/svJ are more susceptible than other species.
Fig.1 Severe crescent formation in a rat model of anti-glomerular basement membrane (GBM) nephritis. (Hewitson et al. 2009)
Application of the Anti-GBM Model
Anti-GBM model has proved to be useful in clarifying the underlying mechanisms and molecular pathogenesis of human glomerulonephritis disease as well as assessing potential novel therapies for renal diseases. Additionally, the anti-GBM model provides a unique approach for rapidly screening agents with potential therapeutic efficacy for other more protracted varieties of immune-mediated nephritis such as lupus nephritis.
Creative Biolabs provides all kinds of assessments for testing the effectiveness of potential drugs depending on your research objectives, which including but not limited to:
Meanwhile, Creative Biolabs also offers other types of rodent urological disease models that you may be interested in:
Creative Biolabs has sufficient expertise in the development, production, and distribution of animal models. We provide a number of rodent models for the assessment of agents designed to treat the urological disease as well as for the study of pathogenesis and complications of diseases. Please contact us to discuss your requirements and experience the value of our services.
Reference
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