Protein biopharmaceuticals, such as monoclonal antibodies (mAbs) are widely studied in drug discovery field for the prevention and treatment of various diseases. As a leading service provider that focuses on antibody for over ten years, Creative Biolabs has been constantly delivering high-quality antibody isoform analysis that are supported by our state-of-art technical platform and rich expertise. From antibody structure, post-translational modifications (PTMs) analysis to antibody aggregation analysis, we succeed with excellence and confidence to meet your demand for streamlining characterization of antibody isoform, which are efficient and cost-effectively.
Antibody Isoform Analysis
Antibodies exist in various isoforms and the various isoforms differ in their biological features, structure, target specificity and distribution. The complex and lengthy upstream and downstream production methods of the antibodies make them susceptible to physical and chemical modifications. As such, the assessment of the antibody isoform provides useful insight into the complex humoral immune response. There are various techniques could be used to analysis the isolated isoforms.
MALDI is a technique for soft ionization of mass spectrometry (MS) that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. Creative Biolabs has briefly introduced the MALDI to help you understand more about your antibody drug candidate. We have the professional MS platform, which contains advanced instruments. By using MS, we can have a deeper and more detailed understanding of the antibody isoform. For instance, the MALDI analysis of the IgG1 product indicates that most of the N-linked glycans were complex, biantennary glycans with core fucosylation. Small amounts of triantennary and high-man-nose glycans were also observed. A significant proportion of the N-glycans contain terminal α-gal and N-glycolyl neuraminic acid (NGNA) residues
Fig.1 Negative-ion MALDI spectrum of N-linked glycans released from IgG1 product. (Sundaram, 2011)
Multiple charge isoforms of mAb could be separated by cation-exchange chromatography, and their identities will be established primarily by ESI-MS analysis of the intact, reduced, and deglycosylated molecule. According to our experience, ESI-MS analysis, an efficient and speedy process, was shown to play an invaluable role in making characterization and structure elucidation.
Along with years of rich experience in the field of drug discovery, Creative Biolabs has successfully finished lots of projects for our customers all over the world. We are now confident in offering the customized proposal to meet your specific needs. If you are interested in our antibody isoform analysis service, please do not hesitate to contact us for more details.
Reference
For Research Use Only.
