Apoptosis Detection Kit, Hoechst 33342 & PI (CAT#: DR-LX014)

Datasheet
Overview
DescriptionCreative Biolabs offers a wide range of dye reagents and kits suitable for evaluating cell biological activity such as apoptosis, endocytosis and can be used in a number of areas: drug discovery, toxicity testing and immunology. Apoptosis Detection Kit, Hoechst 33342 & PI is used for identification and enumeration of dead cells, such as apoptotic or necrotic cells, by FC, IF.
FeaturesConvenient, and simple steps;
Be compatible with various antibody function study platforms;
Reduced signal variability and improved timing in sensitive experiments;
ApplicationsBe used for the identification and enumeration of dead cells, such as apoptotic or necrotic cells, by flow cytometry.
Molecular Mechanism of ActionKit has been developed for detection and discrimination of apoptotic, necrotic, and dead cells. In principle, apoptotic cells are stained positively for Annexin V conjugation that binds to phosphotidylserine (PS), but are negative for staining with propidium iodide (PI). Dead cells are stained positive for Annexin V conjugation and PI, whereas viable cells are negative for both Annexin V conjugation and PI.
Assay MeasuresFluorescence
Research AreasDrug Discovery and Toxicity Testing; Immunology
Specification
Size1 kit
ComponentsHoechst 33342 solution;
Propidium Iodide (PI) solution;
StorageStore protected from light at 2-8 °C. Do not freeze.
HandlingPlease place the product at proper storage condition once received.
ShippingIce packs

For Research Use Only.

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