| Overview | |
|---|---|
| Description | The kit is a high-throughput screening assay that measures the inhibitory activity of small molecules on the binding of BCLXL to its ligand. It is performed in a convenient 384-well plate format. |
| Features | Ready-used for apoptosis assay. |
| Applications | Kit is used to screen compound and study to measure compound binding to the target. |
| Application Notes | Excellent for screening small molecule inhibitors in pharmaceutical research and high-throughput screening. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 384 tests |
| Components | The kit is supplied with an assay kit including enough recombinant BCL-xL, BCL-xL peptide ligand, assay buffer, Anti-His Terbium-Labeled Donor, and Dye-labeled Streptavidin Acceptor for 384 reactions |
| Target | BCL-xL |
| Target/Description | This FRET-based assay is designed for high throughput screening with no need for washing steps. The procedure involves incubating a sample containing donor and acceptor dyes, BCL-XL, peptide ligand, and an inhibitor for 120 minutes. The fluorescence intensity is then measured using a fluorescence reader to assess BCL-XL activity. |
| Format | Supplied with a TR-FRET assay kit. |
| Storage | Store at the appropriate temparature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
