PathSpecific™ Human BCL-2 Assay Kit, TR-FRET (CAT#: ZL-0124-WR31)
| Overview | |
|---|---|
| Description | The kit is a rapid and user-friendly assay for detecting the inhibitory activity of compounds against BCL-2 binding in a homogeneous reaction format. |
| Features | Ready-used for inhibitors screening. |
| Applications | Kit is used to screen compound and study to measure compound binding to the target. |
| Application Notes | Ideal for screening small molecule inhibitors in drug development and high-throughput testing. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with an assay kit including enough recombinant BCL-2, BCL-2 peptide ligand, assay buffer, Anti-His Terbium-Labeled Donor, and Dye-labeled Streptavidin Acceptor for 96 reactions. |
| Target | BCL2 |
| Target/Description | The assay utilizes a Terbium-labeled anti-His antibody that binds to His-tagged BCL-2. A biotin-labeled BCL-2 peptide ligand then allows dye-labeled streptavidin to bind. Proximity-induced TR-FRET signal is generated upon interaction of BCL-2 with the BCL-2 peptide ligand. Fluorescence intensity is measured using a TR-FRET capable fluorescence reader, providing a quantitative measurement of BCL-2 activity. |
| Format | Supplied with a TR-FRET assay kit. |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
Online Inquiry