PathSpecific™ Caspase-1 Assay Kit, Colorimetric (CAT#: ZL-1122-WR6)
| Overview | |
|---|---|
| Description | The kit is used to quantitatively measure the activity of Caspase 1, a protease involved in inflammatory and apoptotic processes, using a YVAD-specific substrate in biological samples. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Caspase 1 protease in cells. |
| Application Notes | The kit is designed for the quantitative determination of caspase activity in samples, making it a valuable tool for studying apoptosis and drug discovery research. |
| Molecular Mechanism of Action | The kit relies on the chromophore p-nitroanilide (pNA) being detected by spectrophotometry following its cleavage from the labeled substrate YVAD-pNA. A spectrophotometer or a microtiter plate reader can be used to measure the pNA light emission at 400 or 405 nm. Caspase-1 activity fold increase may be calculated by comparing the absorbance of pNA from a treated sample to that of an untreated control. |
| Assay Measures | Colorimetric-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 25 tests |
| Components | The kit is supplied with an assay kit including 2X reaction buffer, cell lysis buffer, dilution buffer, DTT, and YVAD-pNA. |
| Target | Caspase 1 |
| Format | Supplied with colorimetric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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