| Overview | |
|---|---|
| Description | The kit is designed for quantifying the enzymatic activity of caspase 2 in tissue extracts and cell lysates. |
| Features | Ready-used for protease assay |
| Applications | Kit is used to study the Caspase 2 protease in biological samples. |
| Application Notes | This kit enables the in vitro quantitative measurement of caspase 2 activity in serum, plasma, and other biological fluids. |
| Molecular Mechanism of Action | Our Caspase-2 Assay Kit, Colorimetric offers a simple and convenient method for assessing the activity of caspases 2. It is based on the cleavage of a labeled substrate by Caspase-2 protease, resulting in the release of p-nitroaniline (pNA). The concentration of pNA, which has a maximum absorbance at 405 nm, can be measured using a microplate reader or spectrophotometer. This allows for the calculation of caspase-2 activity through absorbance values or a standard calibration curve prepared with the pNA Dye Standard. |
| Assay Measures | Colorimetric-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 50 tests;100 tests |
| Components | The kit is supplied with an assay kit including caspase lysis buffer, caspase assay buffer, Ac-VDVAD-pNA substrate, pNA dye standard, and caspase inhibitor Z-VAD-FMK. |
| Target | Caspase 2 |
| Format | Supplied with colorimetric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
