| Overview | |
|---|---|
| Description | The kit is designed for detecting and quantifying the enzyme activity of caspase 3 in cell lysate and tissue extracts. |
| Features | Ready-used for protease assay |
| Applications | Kit is used to study the Caspase 3 protease in biological samples. |
| Application Notes | The kit offers a straightforward and convenient approach to quantitatively measure the activity of caspase enzymes that specifically recognize the DEVD sequence. |
| Molecular Mechanism of Action | This assay method works by creating the chromophore p-nitroaniline (pNA) when it is separated from the labelled substrate, DEVD-pNA. This formed pNA can then be measured with the use of a spectrophotometer or a microplate reader, both of which can detect its absorption at wavelengths of 400 or 405 nm. |
| Assay Measures | Colorimetric-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including 2X reaction buffer, cell lysis buffer, DEVD-pNA, dilution buffer, and DTT. |
| Target | Caspase 3 |
| Format | Supplied with colorimetric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
