PathSpecific™ Caspase-4 Assay Kit, Colorimetric (CAT#: ZL-1122-WR9)
| Overview | |
|---|---|
| Description | The kit is designed to quantify the enzyme activity of caspase 4 in cell lysate and tissue extracts. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Caspase 4 protease in biological samples. |
| Application Notes | The kit offers a user-friendly and efficient method for quantifying the activity of caspases that target the LEVD sequence. |
| Molecular Mechanism of Action | The assay operates by generating the chromophore p-nitroaniline (pNA) from the cleavage of the tagged substrate LEVD-pNA. The content of p-NA can be measured using a spectrophotometer or a microplate reader that detects absorbance at wavelengths of 400 or 405 nm. |
| Assay Measures | Colorimetric-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including 2X reaction buffer, cell lysis buffer, LEVD-pNA, dilution buffer, and DTT. |
| Target | Caspase 4 |
| Format | Supplied with colorimetric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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