PathSpecific™ Caspase 5 Assay Kit, Fluorometric (CAT#: ZL-1122-WR10)
| Overview | |
|---|---|
| Description | The kit can be utilized to assess the enzyme activity of caspase 5 in cellular lysates and tissue extracts. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Caspase 5 protease in biological samples. |
| Application Notes | The kit provides a simple and convenient method for assaying the activity of caspases that recognize the sequence WEHD, allowing for quantification of caspase activity in various biological samples. |
| Molecular Mechanism of Action | The substrate used in the assay, WEHD-AFC, allows spectrophotometric detection by emitting blue light (peak wavelength of 400 nm). Once cleaved by caspase 5 or similar substances, the AFC molecule releases a yellow-green fluorescence (peak wavelength of 505 nm). This emission can be measured using a fluorometer or a fluorecence microplate reader. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including 2X reaction buffer, WEHD-AFC (1mM), cell lysis buffer, dilution buffer, and DTT. |
| Target | Caspase 5 |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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