| Overview | |
|---|---|
| Description | The kit is used to analyze the caspase 8 activity in serum, plasma, and other biological samples. |
| Features | Ready-used for protease assay |
| Applications | Kit is used to study the Caspase 8 protease in biological samples. |
| Application Notes | The kit provides a simple and convenient method to accurately measure the activity of caspase 8 in cell lysates, utilizing the recognition of the Ile-Glu-Thr-Asp (IETD) sequence. |
| Molecular Mechanism of Action | The assay measures Caspase 8 activity by detecting the cleavage of the substrate IETD-pNA, resulting in the release of p-nitroanilide (pNA) that can be quantified using a spectrophotometer or a microtiter plate reader at OD 400 - 405 nm. Comparing the absorbance of pNA between apoptotic and control samples enables the determination of the fold increase in Caspase 8 activity. |
| Assay Measures | Colorimetric-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including 2X reaction buffer, cell lysis buffer, dilution buffer, DTT, and IETD-pNA. |
| Target | Caspase 8 |
| Format | Supplied with colorimetric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
