| Overview | |
|---|---|
| Description | The kit is effective in measuring the enzyme activity of caspase 9 in cell lysate, tissue extracts, and other biological samples. |
| Features | Ready-used for protease assay |
| Applications | Kit is used to study the Caspase 9 protease in biological samples. |
| Application Notes | The assay is based on fluorescence and employs the preferred caspase 9 substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). |
| Molecular Mechanism of Action | The assay is based on fluorometric detection of the cleavage of substrate LEHD-AFC by caspase-9, resulting in the release of AFC. AFC emits a yellow-green fluorescence when cleaved, which can be measured using a fluorometer or fluorescence microtiter plate reader. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including 2X reaction buffer, cell lysis buffer, DTT, and LEHD-AFC (1mM). |
| Target | Caspase 9 |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
