PathSpecific™ Cathepsin B Activity Assay Kit, Fluorometric (CAT#: ZL-1122-WR13)
| Overview | |
|---|---|
| Description | The kit can be effectively used to quantitatively measure the activity of cathepsin B in cell lysate and tissue extracts. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Cathepsin B protease in biological samples. |
| Application Notes | The kit is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). |
| Molecular Mechanism of Action | Upon incubation with the cathepsin-B enzyme, the synthetic substrate RR-AFC is cleaved, resulting in the release of free AFC. The amount of AFC can be measured using a fluorometer or fluorescence plate reader at an excitation wavelength of 400 nm and an emission wavelength of 505 nm. This allows for quantification of cathepsin-B activity in cell lysates or other samples. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including cathepsin B cell lysis buffer, cathepsin inhibitor, cathepsin B reaction buffer, and cathepsin B substrate. |
| Target | Cathepsin B |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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